α-interferon treatment induces quantitative modifications of HLA class I-associated peptides eluted from cultured cancer cell lines

G. Cangemi, E. Millo, G. Damonte, C. Semino, G. Pietra, U. Benatti, Giovanni Melioli

Research output: Contribution to journalArticle

Abstract

Interferons upregulate the expression of HLA class I antigens on cancer cells. Nevertheless little is known about the panel of HLA class I antigen-associated peptides presented by recombinant α-interferon (rα-IFN)-treated cells. For this reason, peptides were eluted from five cancer cell lines (four melanoma and one non-small cell lung cancer) following treatment with rα-IFN. High-performance liquid chromatography (HPLC) profiles of the peptide fractions were compared with those obtained from untreated cells. No significant differences in peptide characteristics (detectable on the basis of retention times) were observed, but significant differences in terms of peptide quantities were observed. Mass spectrometry performed on HPLC peaks allowed not only the detection of three different peptides (two derived from the MAGE family of genes and one from the mart-1) both in untreated and in treated cells, but also gave an indication of the number of peptides within one HPLC peak. This data demonstrates that rα-IFN-treated cells express a similar peptide pattern as untreated cells, with significant quantitative differences. Interestingly, this fmding also explains the higher susceptibility to lysis (mediated by specific cytolytic lymphocytes, which recognize cancer cells in an HLA-restricted fashion) of rα-IFN-treated cells.

Original languageEnglish
Pages (from-to)212-218
Number of pages7
JournalTissue Antigens
Volume55
Issue number3
DOIs
Publication statusPublished - 2000

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Keywords

  • α-interferon
  • High-performance liquid chromatography
  • HLA class I
  • Mass-spectrometry
  • Peptides

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

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