α-melanocyte-stimulating hormone protects the allograft in experimental heart transplantation

Stefano Gatti, Gualtiero Colombo, Roberto Buffa, Flavia Turcatti, Letizia Garofalo, Nadia Carboni, Luca Ferla, Luigi R. Fassati, James M. Lipton, Anna Catania

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Background. With the increasing need for organ transplantation and the use of "marginal" organs, novel approaches are sought to increase the efficiency and survival of transplanted tissue. We tested the idea that treatment with the anti-inflammatory peptide, α-melanocyte-stimulating hormone (α-MSH), an endogenous hormone that does not cause marked immunosuppression but does reduce reperfusion injury, may protect allografts and prolong their survival. Methods. Donor cardiac grafts (Brown Norway) were transplanted heterotopically into the abdomen of recipient (Lewis) rats. Treatments consisted of intraperitoneal injections of Nle4DPhe7-α-MSH (NDP-α-MSH) or saline from the time of transplantation until sacrifice or spontaneous rejection. Allografts were removed on day 1, day 4, or at the time of rejection and examined for histopathology and expression of molecules prominent in reperfusion injury, transplant rejection, and apoptosis. Results. NDP-α-MSH treatment caused a significant increase in allograft survival and a marked decrease in leukocyte infiltration. Expression of molecules such as endothelin 1, chemokines, and adhesion molecules, which are involved in allograft rejection, was significantly inhibited in NDP-α-MSH-treated rats. Conclusions. The results indicate that protection of the allograft from early injury with α-MSH can postpone rejection. Addition of this early protection with the peptide to usual treatment with immunosuppressive agents may, therefore, improve success of organ transplants.

Original languageEnglish
Pages (from-to)1678-1684
Number of pages7
JournalTransplantation
Volume74
Issue number12
Publication statusPublished - Dec 27 2002

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Melanocyte-Stimulating Hormones
Heart Transplantation
Allografts
Reperfusion Injury
Transplants
Tissue Survival
Peptides
Graft Rejection
Organ Transplantation
Endothelin-1
Therapeutics
Norway
Immunosuppressive Agents
Intraperitoneal Injections
Chemokines
Abdomen
Immunosuppression
Leukocytes
Anti-Inflammatory Agents
Transplantation

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

Gatti, S., Colombo, G., Buffa, R., Turcatti, F., Garofalo, L., Carboni, N., ... Catania, A. (2002). α-melanocyte-stimulating hormone protects the allograft in experimental heart transplantation. Transplantation, 74(12), 1678-1684.

α-melanocyte-stimulating hormone protects the allograft in experimental heart transplantation. / Gatti, Stefano; Colombo, Gualtiero; Buffa, Roberto; Turcatti, Flavia; Garofalo, Letizia; Carboni, Nadia; Ferla, Luca; Fassati, Luigi R.; Lipton, James M.; Catania, Anna.

In: Transplantation, Vol. 74, No. 12, 27.12.2002, p. 1678-1684.

Research output: Contribution to journalArticle

Gatti, S, Colombo, G, Buffa, R, Turcatti, F, Garofalo, L, Carboni, N, Ferla, L, Fassati, LR, Lipton, JM & Catania, A 2002, 'α-melanocyte-stimulating hormone protects the allograft in experimental heart transplantation', Transplantation, vol. 74, no. 12, pp. 1678-1684.
Gatti S, Colombo G, Buffa R, Turcatti F, Garofalo L, Carboni N et al. α-melanocyte-stimulating hormone protects the allograft in experimental heart transplantation. Transplantation. 2002 Dec 27;74(12):1678-1684.
Gatti, Stefano ; Colombo, Gualtiero ; Buffa, Roberto ; Turcatti, Flavia ; Garofalo, Letizia ; Carboni, Nadia ; Ferla, Luca ; Fassati, Luigi R. ; Lipton, James M. ; Catania, Anna. / α-melanocyte-stimulating hormone protects the allograft in experimental heart transplantation. In: Transplantation. 2002 ; Vol. 74, No. 12. pp. 1678-1684.
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