α1-Adrenergic stimulation of in vitro growth hormone release and cytosolic free Ca2+ in rat somatotrophs

A. Pandiella, F. R. Elahi, L. Vallar, A. Spada

Research output: Contribution to journalArticlepeer-review

Abstract

The effects of α1-adrenergic agents on GH release and intracellular free Ca2+ concentration ([Ca2+]i) were investigated in purified rat somatotroph preparations. Phenylephrine (PHE) stimulated in vitro GH release; the maximal effect (2.5-fold stimulation) occurred at 1 αM PHE. The effect was completely blocked by the α-adrenergic antagonist phentolamine and partially counteracted by the β-antagonist propranolol. Experiments with the fluorescent Ca2+ probe fura 2 show that PHE causes [Ca2+]i to rise from 178 ± 31 nM (mean ± SE; n = 25) to 370 ± 55 nM (n = 9). This effect was complete within 20 sec and was maintained for at least 5-10 min. The rise was rapidly interrupted by administration of 1 μM phentolamine. The β-receptor agonist isoproterenol caused a small [Ca2+]i rise due to action on α1-adrenoreceptors. The PHE-induced [Ca2+]i rise showed two components: an initial peak due to Ca2+ mobilization from intracellular stores and a subsequent rise due to Ca2+ influx from the extracellular space. Somatostatin (SRIF) lowered both resting [Ca2+]i and Ca2+ influx stimulated by PHE. Pertussis toxin pretreatment did not modify PHE-induced [Ca2+]i changes, while it completely prevented the effect of SRIF on both resting and triggered [Ca2+]i, thus suggesting that a GTP-binding protein sensitive to the toxin is involved in the transduction of SRIF action. The increase in cAMP induced by cholera toxin pretreatment modified neither PHE nor SRIF action on [Ca2+]i. In conclusion on [Ca2+]i. In conclusion, in rat somatotrophs Ca2+ mobilization and influx are stimulated by α1-adrenergic agents, and this triggered [Ca2+]i rise results in a stimulation of GH release. In these cells SRIF is able to reduce both resting [Ca2+]i levels and [Ca2+]i increases induced by α1-adrenergic activation.

Original languageEnglish
Pages (from-to)1419-1425
Number of pages7
JournalEndocrinology
Volume122
Issue number4
Publication statusPublished - 1988

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

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