α1-antitrypsin in serum determined by capillary isoelectric focusing

Anna Lupi, Simona Viglio, Maurizio Luisetti, Marina Gorrini, Pierpaolo Coni, Gavino Faa, Giuseppe Cetta, Paolo Iadarola

Research output: Contribution to journalArticlepeer-review

Abstract

A capillary isoelectric focusing (CIEF) method using bare fused-silica capillaries filled with polyethylene oxide (PEO) and carrier ampholyte solutions in the pH 3.5-5.0 range has been developed for the identification of alpha1-antitrypsin (α1AT) phenotypes in human serum. This novel procedure was routinely applied to the study of serum samples of five controls whose α1AT phenotype was previously identified and of twelve subjects whose α1AT phenotype was unknown. The results obtained allowed us to confirm or identify the α1AT phenotype in all sera tested. This procedure seems particularly suitable for identification of α1AT variants associated with diseases of clinical relevance.

Original languageEnglish
Pages (from-to)3318-3326
Number of pages9
JournalElectrophoresis
Volume21
Issue number15
DOIs
Publication statusPublished - 2000

Keywords

  • α-antitrypsin phenotypes
  • Capillary isoelectric focusing
  • Polyethylene oxide

ASJC Scopus subject areas

  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'α1-antitrypsin in serum determined by capillary isoelectric focusing'. Together they form a unique fingerprint.

Cite this