α1-antitrypsin in serum determined by capillary isoelectric focusing

Anna Lupi; Simona Viglio; Maurizio Luisetti; Marina Gorrini; Pierpaolo Coni; Gavino Faa; Giuseppe Cetta; Paolo Iadarola

doi:10.1002/1522-2683(20000901)21:15<3318::AID-ELPS3318>3.0.CO;2-I

α1-antitrypsin in serum determined by capillary isoelectric focusing

Anna Lupi, Simona Viglio, Maurizio Luisetti, Marina Gorrini, Pierpaolo Coni, Gavino Faa, Giuseppe Cetta, Paolo Iadarola

IRCCS Fondazione Policlinico San Matteo

Research output: Contribution to journal › Article › peer-review

Abstract

A capillary isoelectric focusing (CIEF) method using bare fused-silica capillaries filled with polyethylene oxide (PEO) and carrier ampholyte solutions in the pH 3.5-5.0 range has been developed for the identification of alpha₁-antitrypsin (α₁AT) phenotypes in human serum. This novel procedure was routinely applied to the study of serum samples of five controls whose α₁AT phenotype was previously identified and of twelve subjects whose α₁AT phenotype was unknown. The results obtained allowed us to confirm or identify the α₁AT phenotype in all sera tested. This procedure seems particularly suitable for identification of α₁AT variants associated with diseases of clinical relevance.

Original language	English
Pages (from-to)	3318-3326
Number of pages	9
Journal	Electrophoresis
Volume	21
Issue number	15
DOIs	https://doi.org/10.1002/1522-2683(20000901)21:15<3318::AID-ELPS3318>3.0.CO;2-I
Publication status	Published - 2000

Keywords

α-antitrypsin phenotypes
Capillary isoelectric focusing
Polyethylene oxide

ASJC Scopus subject areas

Clinical Biochemistry

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10.1002/1522-2683(20000901)21:15<3318::AID-ELPS3318>3.0.CO;2-I

Cite this

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title = "α1-antitrypsin in serum determined by capillary isoelectric focusing",

abstract = "A capillary isoelectric focusing (CIEF) method using bare fused-silica capillaries filled with polyethylene oxide (PEO) and carrier ampholyte solutions in the pH 3.5-5.0 range has been developed for the identification of alpha1-antitrypsin (α1AT) phenotypes in human serum. This novel procedure was routinely applied to the study of serum samples of five controls whose α1AT phenotype was previously identified and of twelve subjects whose α1AT phenotype was unknown. The results obtained allowed us to confirm or identify the α1AT phenotype in all sera tested. This procedure seems particularly suitable for identification of α1AT variants associated with diseases of clinical relevance.",

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T1 - α1-antitrypsin in serum determined by capillary isoelectric focusing

AU - Lupi, Anna

AU - Viglio, Simona

AU - Luisetti, Maurizio

AU - Gorrini, Marina

AU - Coni, Pierpaolo

AU - Faa, Gavino

AU - Cetta, Giuseppe

AU - Iadarola, Paolo

PY - 2000

Y1 - 2000

N2 - A capillary isoelectric focusing (CIEF) method using bare fused-silica capillaries filled with polyethylene oxide (PEO) and carrier ampholyte solutions in the pH 3.5-5.0 range has been developed for the identification of alpha1-antitrypsin (α1AT) phenotypes in human serum. This novel procedure was routinely applied to the study of serum samples of five controls whose α1AT phenotype was previously identified and of twelve subjects whose α1AT phenotype was unknown. The results obtained allowed us to confirm or identify the α1AT phenotype in all sera tested. This procedure seems particularly suitable for identification of α1AT variants associated with diseases of clinical relevance.

AB - A capillary isoelectric focusing (CIEF) method using bare fused-silica capillaries filled with polyethylene oxide (PEO) and carrier ampholyte solutions in the pH 3.5-5.0 range has been developed for the identification of alpha1-antitrypsin (α1AT) phenotypes in human serum. This novel procedure was routinely applied to the study of serum samples of five controls whose α1AT phenotype was previously identified and of twelve subjects whose α1AT phenotype was unknown. The results obtained allowed us to confirm or identify the α1AT phenotype in all sera tested. This procedure seems particularly suitable for identification of α1AT variants associated with diseases of clinical relevance.

KW - α-antitrypsin phenotypes

KW - Capillary isoelectric focusing

KW - Polyethylene oxide

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ER -

α1-antitrypsin in serum determined by capillary isoelectric focusing

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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