α2(I) collagen gene expression is up-regulated in quail chondrocytes pretreated with retinoic acid

M. Sanchez, E. Gionti, A. Arcella, G. Pontarelli, F. De Lorenzo

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

α2(I) collagen gene expression is induced in quail embryo chondrocytes pretreated with retinoic acid (RA). The initial appearance of α2(I) mRNA occurs around day 3 of culture in RA-free medium and rapidly progresses over the next 4 days. In transient transfection assays, expression of COL1A2-CAT, a chimeric gene bearing 3500 bp upstream the bone/tendon transcription start site from the human α2(I) gene fused to the CAT gene, is stimulated severalfold in RA-treated chondrocytes. In contrast, enzyme activity is very low in untreated chondrocytes, suggesting that the sequences required for RA-induced transcription of the α2(I) gene are present in this plasmid. Analysis of α2(I) promoter sequences performed with deletion mutants gives overlapping results in collagen type I-producing fibroblasts and chondrocytes withdrawn from RA treatment. These experiments suggest that RA-induced transcription of the α2(I) collagen gene in chondrocytes is regulated by the binding of transcription factors to the same regulatory sequences that control transcription in fibroblasts.

Original languageEnglish
Pages (from-to)115-119
Number of pages5
JournalBiochemical Journal
Volume295
Issue number1
Publication statusPublished - 1993

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Quail
Chondrocytes
Tretinoin
Gene expression
Collagen
Gene Expression
Genes
Transcription
Fibroblasts
Bearings (structural)
Transcription Initiation Site
Tendons
Enzyme activity
Collagen Type I
Transfection
Assays
Bone
Plasmids
Transcription Factors
Embryonic Structures

ASJC Scopus subject areas

  • Biochemistry

Cite this

α2(I) collagen gene expression is up-regulated in quail chondrocytes pretreated with retinoic acid. / Sanchez, M.; Gionti, E.; Arcella, A.; Pontarelli, G.; De Lorenzo, F.

In: Biochemical Journal, Vol. 295, No. 1, 1993, p. 115-119.

Research output: Contribution to journalArticle

Sanchez, M, Gionti, E, Arcella, A, Pontarelli, G & De Lorenzo, F 1993, 'α2(I) collagen gene expression is up-regulated in quail chondrocytes pretreated with retinoic acid', Biochemical Journal, vol. 295, no. 1, pp. 115-119.
Sanchez, M. ; Gionti, E. ; Arcella, A. ; Pontarelli, G. ; De Lorenzo, F. / α2(I) collagen gene expression is up-regulated in quail chondrocytes pretreated with retinoic acid. In: Biochemical Journal. 1993 ; Vol. 295, No. 1. pp. 115-119.
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