Abstract
We studied the expression of adhesion molecules affecting recirculation and homing on peripheral blood CD4+ T cells of patients with systemic sclerosis (SSc), in order to evaluate whether the distribution of tissue targeted subsets could reflect the participation of internal organs or the extent of cutaneous involvement [i.e. limited cutaneous (lc) and diffuse cutaneous (dc)]. Peripheral blood mononuclear cells (PBMC) from 51 patients with SSc and 19 sex- and age-matched controls were investigated by cytofluorimetric analysis for lymphocyte subpopulations carrying the following surface molecules: CD3, CD4, CLA, α4β7 and α4β1. Standard routine biochemistry and clinical examinations were also performed in all patients. We found that both α4β1+ and α4β7+ cells within the CD4+ T cell population were significantly increased, while CLA+ CD4+ T cells were significantly reduced in SSc, compared to healthy donors. Significantly lower absolute numbers of α4β7+ cells were found in lc- compared to dc-SSc. Patients with oesophageal involvement had high numbers of α4β7+ cells, while those with nephritis also showed low levels of CLA+ cells. Lung involvement was related directly to α4β1+ cell numbers and inversely to α4β7+ CD4 cell numbers. Taken together, our findings demonstrate that distinct CD4+ T cell populations with selective homing properties show changes from normal distribution in SSc, and such changes are related to clinical expression and organ involvement in the course of the disease.
| Original language | English |
|---|---|
| Pages (from-to) | 551-557 |
| Number of pages | 7 |
| Journal | Clinical and Experimental Immunology |
| Volume | 139 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 2005 |
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Keywords
- α4β1
- α4β7
- CLA
- Systemic sclerosis
ASJC Scopus subject areas
- Immunology
Cite this
α4β1+ and α4β7+ CD4+ T cell numbers increase and CLA+ CD4+ T cell numbers decrease in systemic sclerosis. / Scala, Enrico; Paganelli, R.; Sampogna, F.; Abeni, D.; Colonna, L.; De Pità, O.; Puddu, P.; Russo, G.
In: Clinical and Experimental Immunology, Vol. 139, No. 3, 03.2005, p. 551-557.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - α4β1+ and α4β7+ CD4+ T cell numbers increase and CLA+ CD4+ T cell numbers decrease in systemic sclerosis
AU - Scala, Enrico
AU - Paganelli, R.
AU - Sampogna, F.
AU - Abeni, D.
AU - Colonna, L.
AU - De Pità, O.
AU - Puddu, P.
AU - Russo, G.
PY - 2005/3
Y1 - 2005/3
N2 - We studied the expression of adhesion molecules affecting recirculation and homing on peripheral blood CD4+ T cells of patients with systemic sclerosis (SSc), in order to evaluate whether the distribution of tissue targeted subsets could reflect the participation of internal organs or the extent of cutaneous involvement [i.e. limited cutaneous (lc) and diffuse cutaneous (dc)]. Peripheral blood mononuclear cells (PBMC) from 51 patients with SSc and 19 sex- and age-matched controls were investigated by cytofluorimetric analysis for lymphocyte subpopulations carrying the following surface molecules: CD3, CD4, CLA, α4β7 and α4β1. Standard routine biochemistry and clinical examinations were also performed in all patients. We found that both α4β1+ and α4β7+ cells within the CD4+ T cell population were significantly increased, while CLA+ CD4+ T cells were significantly reduced in SSc, compared to healthy donors. Significantly lower absolute numbers of α4β7+ cells were found in lc- compared to dc-SSc. Patients with oesophageal involvement had high numbers of α4β7+ cells, while those with nephritis also showed low levels of CLA+ cells. Lung involvement was related directly to α4β1+ cell numbers and inversely to α4β7+ CD4 cell numbers. Taken together, our findings demonstrate that distinct CD4+ T cell populations with selective homing properties show changes from normal distribution in SSc, and such changes are related to clinical expression and organ involvement in the course of the disease.
AB - We studied the expression of adhesion molecules affecting recirculation and homing on peripheral blood CD4+ T cells of patients with systemic sclerosis (SSc), in order to evaluate whether the distribution of tissue targeted subsets could reflect the participation of internal organs or the extent of cutaneous involvement [i.e. limited cutaneous (lc) and diffuse cutaneous (dc)]. Peripheral blood mononuclear cells (PBMC) from 51 patients with SSc and 19 sex- and age-matched controls were investigated by cytofluorimetric analysis for lymphocyte subpopulations carrying the following surface molecules: CD3, CD4, CLA, α4β7 and α4β1. Standard routine biochemistry and clinical examinations were also performed in all patients. We found that both α4β1+ and α4β7+ cells within the CD4+ T cell population were significantly increased, while CLA+ CD4+ T cells were significantly reduced in SSc, compared to healthy donors. Significantly lower absolute numbers of α4β7+ cells were found in lc- compared to dc-SSc. Patients with oesophageal involvement had high numbers of α4β7+ cells, while those with nephritis also showed low levels of CLA+ cells. Lung involvement was related directly to α4β1+ cell numbers and inversely to α4β7+ CD4 cell numbers. Taken together, our findings demonstrate that distinct CD4+ T cell populations with selective homing properties show changes from normal distribution in SSc, and such changes are related to clinical expression and organ involvement in the course of the disease.
KW - α4β1
KW - α4β7
KW - CLA
KW - Systemic sclerosis
UR - http://www.scopus.com/inward/record.url?scp=14744285822&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=14744285822&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2249.2005.02729.x
DO - 10.1111/j.1365-2249.2005.02729.x
M3 - Article
C2 - 15730402
AN - SCOPUS:14744285822
VL - 139
SP - 551
EP - 557
JO - Clinical and Experimental Immunology
JF - Clinical and Experimental Immunology
SN - 0009-9104
IS - 3
ER -