αV integrins in Schwann cells promote attachment to axons, but are dispensable in vivo

Kathleen K. Catignas, Luciana R. Frick, Marta Pellegatta, Edward Hurley, Zachary Kolb, Kathryn Addabbo, Joseph H. McCarty, Richard O. Hynes, Arjan van der Flier, Yannick Poitelon, Lawrence Wrabetz, Maria Laura Feltri

Research output: Contribution to journalArticlepeer-review

Abstract

In the developing peripheral nervous system, Schwann cells (SCs) extend their processes to contact, sort, and myelinate axons. The mechanisms that contribute to the interaction between SCs and axons are just beginning to be elucidated. Using a SC-neuron coculture system, we demonstrate that Arg-Gly-Asp (RGD) peptides that inhibit αV-containing integrins delay the extension of SCs elongating on axons. αV integrins in SC localize to sites of contact with axons and are expressed early in development during radial sorting and myelination. Short interfering RNA-mediated knockdown of the αV integrin subunit also delays SC extension along axons in vitro, suggesting that αV-containing integrins participate in axo-glial interactions. However, mice lacking the αV subunit in SCs, alone or in combination with the potentially compensating α5 subunit, or the αV partners β3 or β8, myelinate normally during development and remyelinate normally after nerve crush, indicating that overlapping or compensatory mechanisms may hide the in vivo role of RGD-binding integrins.

Original languageEnglish
Pages (from-to)91-108
Number of pages18
JournalGLIA
Volume69
Issue number1
DOIs
Publication statusPublished - Jan 2021

Keywords

  • axo-glial interactions
  • integrin
  • lamellipodia
  • process extension
  • RGD
  • Schwann cell

ASJC Scopus subject areas

  • Neurology
  • Cellular and Molecular Neuroscience

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