β-Adrenergic agonist infusion during extracorporeal lung perfusion: Effects on glucose concentration in the perfusion fluid and on lung function

Franco Valenza, Lorenzo Rosso, Silvia Coppola, Sara Froio, Jacopo Colombo, Roberto Dossi, Jacopo Fumagalli, Valentina Salice, Marta Pizzocri, Grazia Conte, Stefano Gatti, Luigi Santambrogio, Luciano Gattinoni

Research output: Contribution to journalArticle

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Abstract

Background: We recently showed in a pig model of ex vivo lung perfusion (EVLP) that lung edema correlates with glucose consumption. We investigated whether salbutamol, a β-adrenergic receptor agonist known to upregulate fluid transport in the lung, modulates glucose concentration in the perfusate during EVLP. Methods: Lungs from domestic pigs underwent normothermic EVLP. At the end of controlled reperfusion, lungs were ventilated and perfused for 60 minutes, then randomized to salbutamol (β-Agonist) infusion or placebo (Control) for 180 minutes. Functional parameters were assessed. Results: In the β-Agonist group, glucose concentration decreased over time more than corresponding Control values (analysis of variance [ANOVA], p = 0.05). Mean pulmonary artery pressure (mPAP) was 16 ± 1 mm Hg in the β-Agonist group vs 21 ± 1 mm Hg in the Controls (ANOVA p <0.05). Baseline mPAP was correlated with the drop of mPAP after the β-agonist infusion (R 2 = 0.856, p <0.05). Dynamic compliance dropped from 51 ± 10 to 31 ± 6 ml/cm H 2O in the β-Agonist group and from 60 ± 4 to 21 ± 3 ml/cm H 2O in the Control group (ANOVA, p <0.05 β-agonist vs Control). The Δ partial pressure of oxygen/fraction of inspired oxygen was 418 ± 15 and 393 ± 12 mm Hg in the β-Agonist and Control groups, respectively (t-test p = 0.106). Conclusions: Glucose concentration in the perfusate was affected by salbutamol. Salbutamol was associated with lower pulmonary pressures and better lung mechanics. These data suggest a possible role for salbutamol as a pharmacologic adjunct during EVLP before transplantation.

Original languageEnglish
Pages (from-to)524-530
Number of pages7
JournalJournal of Heart and Lung Transplantation
Volume31
Issue number5
DOIs
Publication statusPublished - May 2012

Fingerprint

Adrenergic Agonists
Perfusion
Glucose
Lung
Albuterol
Pulmonary Artery
Pressure
Analysis of Variance
Oxygen
Sus scrofa
Control Groups
Partial Pressure
Mechanics
Compliance
Reperfusion
Edema
Up-Regulation
Swine
Transplantation
Placebos

Keywords

  • β-agonist
  • EVLP
  • Ex-vivo lung perfusion
  • glucose
  • lung edema
  • lung metabolism

ASJC Scopus subject areas

  • Transplantation
  • Cardiology and Cardiovascular Medicine
  • Pulmonary and Respiratory Medicine
  • Surgery

Cite this

β-Adrenergic agonist infusion during extracorporeal lung perfusion : Effects on glucose concentration in the perfusion fluid and on lung function. / Valenza, Franco; Rosso, Lorenzo; Coppola, Silvia; Froio, Sara; Colombo, Jacopo; Dossi, Roberto; Fumagalli, Jacopo; Salice, Valentina; Pizzocri, Marta; Conte, Grazia; Gatti, Stefano; Santambrogio, Luigi; Gattinoni, Luciano.

In: Journal of Heart and Lung Transplantation, Vol. 31, No. 5, 05.2012, p. 524-530.

Research output: Contribution to journalArticle

Valenza, Franco ; Rosso, Lorenzo ; Coppola, Silvia ; Froio, Sara ; Colombo, Jacopo ; Dossi, Roberto ; Fumagalli, Jacopo ; Salice, Valentina ; Pizzocri, Marta ; Conte, Grazia ; Gatti, Stefano ; Santambrogio, Luigi ; Gattinoni, Luciano. / β-Adrenergic agonist infusion during extracorporeal lung perfusion : Effects on glucose concentration in the perfusion fluid and on lung function. In: Journal of Heart and Lung Transplantation. 2012 ; Vol. 31, No. 5. pp. 524-530.
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AU - Valenza, Franco

AU - Rosso, Lorenzo

AU - Coppola, Silvia

AU - Froio, Sara

AU - Colombo, Jacopo

AU - Dossi, Roberto

AU - Fumagalli, Jacopo

AU - Salice, Valentina

AU - Pizzocri, Marta

AU - Conte, Grazia

AU - Gatti, Stefano

AU - Santambrogio, Luigi

AU - Gattinoni, Luciano

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KW - lung edema

KW - lung metabolism

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