β-Adrenergic receptor kinase-1 levels in catecholamine-induced myocardial hypertrophy: Regulation by β- but not α1-adrenergic stimulation

Guido Iaccarino, Paul C. Dolber, Robert J. Lefkowitz, Walter J. Koch

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Pressure overload ventricular hypertrophy is accompanied by dysfunctional β-adrenergic receptor signaling due to increased levels of the β-adrenergic receptor kinase-1, which phosphorylates and desensitizes β- adrenergic receptors. In this study, we examined whether increased β- adrenergic receptor kinase 1 expression is associated with myocardial hypertrophy induced by adrenergic stimulation. With use of implanted mini- osmotic pumps, we treated mice with isoproterenol, phenylephrine, or vehicle to distinguish between α1- and β-adrenergic stimulation. Both treatments resulted in cardiac hypertrophy, but only isoproterenol induced significant increases in β-adrenergic receptor kinase-1 protein levels and activity. Similarly, in isolated adult rat cardiac myocytes, 24 hours of isoproterenol stimulation resulted in a significant 2.8-fold increase in β-adrenergic receptor kinase-1 protein levels, whereas 24 hours of phenylephrine treatment did not alter β-adrenergic receptor kinase-1 expression. Our results indicate that increased β-adrenergic receptor kinase-1 is not invariably associated with myocardial hypertrophy but apparently is controlled by the state of β-adrenergic receptor activation.

Original languageEnglish
Pages (from-to)396-401
Number of pages6
JournalHypertension
Volume33
Issue number1 II
Publication statusPublished - Jan 1999

Fingerprint

Adrenergic Agents
Adrenergic Receptors
Hypertrophy
Catecholamines
Phosphotransferases
Isoproterenol
Phenylephrine
Protein Kinases
Cardiomegaly
Ventricular Pressure
Cardiac Myocytes

Keywords

  • β-Adrenergic receptor
  • Catecholamines
  • Desensitization
  • G protein-coupled receptor kinases
  • Myocardial hypertrophy

ASJC Scopus subject areas

  • Internal Medicine

Cite this

β-Adrenergic receptor kinase-1 levels in catecholamine-induced myocardial hypertrophy : Regulation by β- but not α1-adrenergic stimulation. / Iaccarino, Guido; Dolber, Paul C.; Lefkowitz, Robert J.; Koch, Walter J.

In: Hypertension, Vol. 33, No. 1 II, 01.1999, p. 396-401.

Research output: Contribution to journalArticle

Iaccarino, Guido ; Dolber, Paul C. ; Lefkowitz, Robert J. ; Koch, Walter J. / β-Adrenergic receptor kinase-1 levels in catecholamine-induced myocardial hypertrophy : Regulation by β- but not α1-adrenergic stimulation. In: Hypertension. 1999 ; Vol. 33, No. 1 II. pp. 396-401.
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