Abstract
The effect of a peptide homologous to the biologically active fragment of β amyloid 25-35 (β 25-35) was studied on interleukin 6 (IL-6) and tumour necrosis factor (TNF-α) secretion induced by lipopolysacharide (LPS) in primary rat astrocytes and microglia. Twenty-four hour exposure to LPS (50 ng/ml) induced IL-6 and TNF-α both in astrocytes and in microglial cells, while the effect of β 25-35 (50 μM) per se was negligible in both cell types. In microglial cells, the application of β peptide did not alter the production of either cytokine induced by LPS. However, β 25-35 strongly amplified the production of both IL-6 and TNF-α in astrocytes. These findings confirm the complex interaction between cytokines and amyloidogenesis in Alzheimer's disease and indicate that astrocytes rather than microglia respond to the β amyloid fragment, suggesting that these cells may be actively involved in cytokine-mediated events in AD.
| Original language | English |
|---|---|
| Pages (from-to) | 759-762 |
| Number of pages | 4 |
| Journal | Cytokine |
| Volume | 9 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Oct 1997 |
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Keywords
- β-amyloid
- Alzheimer's disease
- Astrocytes
- IL-6
- TNF
ASJC Scopus subject areas
- Endocrinology
- Molecular Biology
- Immunology
- Immunology and Allergy
Cite this
β-amyloid fragment potentiates IL-6 and TNF-α secretion by LPS in astrocytes but not in microglia. / Forloni, Gianluigi; Mangiarotti, Fabio; Angeretti, Nadia; Lucca, Elisa; De Simoni, Maria Grazia.
In: Cytokine, Vol. 9, No. 10, 10.1997, p. 759-762.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - β-amyloid fragment potentiates IL-6 and TNF-α secretion by LPS in astrocytes but not in microglia
AU - Forloni, Gianluigi
AU - Mangiarotti, Fabio
AU - Angeretti, Nadia
AU - Lucca, Elisa
AU - De Simoni, Maria Grazia
PY - 1997/10
Y1 - 1997/10
N2 - The effect of a peptide homologous to the biologically active fragment of β amyloid 25-35 (β 25-35) was studied on interleukin 6 (IL-6) and tumour necrosis factor (TNF-α) secretion induced by lipopolysacharide (LPS) in primary rat astrocytes and microglia. Twenty-four hour exposure to LPS (50 ng/ml) induced IL-6 and TNF-α both in astrocytes and in microglial cells, while the effect of β 25-35 (50 μM) per se was negligible in both cell types. In microglial cells, the application of β peptide did not alter the production of either cytokine induced by LPS. However, β 25-35 strongly amplified the production of both IL-6 and TNF-α in astrocytes. These findings confirm the complex interaction between cytokines and amyloidogenesis in Alzheimer's disease and indicate that astrocytes rather than microglia respond to the β amyloid fragment, suggesting that these cells may be actively involved in cytokine-mediated events in AD.
AB - The effect of a peptide homologous to the biologically active fragment of β amyloid 25-35 (β 25-35) was studied on interleukin 6 (IL-6) and tumour necrosis factor (TNF-α) secretion induced by lipopolysacharide (LPS) in primary rat astrocytes and microglia. Twenty-four hour exposure to LPS (50 ng/ml) induced IL-6 and TNF-α both in astrocytes and in microglial cells, while the effect of β 25-35 (50 μM) per se was negligible in both cell types. In microglial cells, the application of β peptide did not alter the production of either cytokine induced by LPS. However, β 25-35 strongly amplified the production of both IL-6 and TNF-α in astrocytes. These findings confirm the complex interaction between cytokines and amyloidogenesis in Alzheimer's disease and indicate that astrocytes rather than microglia respond to the β amyloid fragment, suggesting that these cells may be actively involved in cytokine-mediated events in AD.
KW - β-amyloid
KW - Alzheimer's disease
KW - Astrocytes
KW - IL-6
KW - TNF
UR - http://www.scopus.com/inward/record.url?scp=0031258670&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031258670&partnerID=8YFLogxK
U2 - 10.1006/cyto.1997.0232
DO - 10.1006/cyto.1997.0232
M3 - Article
C2 - 9344508
AN - SCOPUS:0031258670
VL - 9
SP - 759
EP - 762
JO - Cytokine
JF - Cytokine
SN - 1043-4666
IS - 10
ER -