Abstract
We investigated β-endorphin (BE) content in an HIV-infected cell line and in peripheral blood mononuclear cells (PBM) from HIV-positive subjects. HIV infection increased BE content in HuT78 cell line compared to uninfected cells. Accordingly, BE content was greater in HIV-positive subjects than in healthy controls, both in fresh PBM and in mitogen-stimulated or unstimulated cultured cells. Further, in PHA-stimulated cultures, BE increase was correlated with disease progression. Opioids are known to decrease immune responsiveness in vivo, and it may be that the increased BE concentrations contribute to HIV-associated immune deficiency. In HIV-positive subjects, but not in healthy controls, intracellular BE concentration was positively correlated with PHA-induced PBM proliferation. The latter data suggest an alternative explanation: that the increased BE content represents a paradoxical response of the host in an attempt to balance virus-induced immunodepression. Thus, BE may be important in fine-tuning of the immune response with its up- and downregulation dependent upon differences in immune status.
| Original language | English |
|---|---|
| Pages (from-to) | 769-775 |
| Number of pages | 7 |
| Journal | Peptides |
| Volume | 15 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1994 |
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Keywords
- Cytokines
- HIV infection
- Opioids
- β-Endorphin
ASJC Scopus subject areas
- Biochemistry
- Endocrinology
- Physiology
- Cellular and Molecular Neuroscience
Cite this
β-Endorphin content in HIV-infected HuT78 cell line and in peripheral lymphocytes from HIV-positive subjects. / Barcellini, Wilma; Sacerdote, Paola; Borghi, Maria Orietta; Rizzardi, Gian Paolo; Fain, Cristina; De Giuli Morghen, Carlo; Manfredi, Barbara; Lazzarin, Adriano; Meroni, Pier Luigi; Panerai, Alberto E.; Zanussi, Carlo.
In: Peptides, Vol. 15, No. 5, 1994, p. 769-775.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - β-Endorphin content in HIV-infected HuT78 cell line and in peripheral lymphocytes from HIV-positive subjects
AU - Barcellini, Wilma
AU - Sacerdote, Paola
AU - Borghi, Maria Orietta
AU - Rizzardi, Gian Paolo
AU - Fain, Cristina
AU - De Giuli Morghen, Carlo
AU - Manfredi, Barbara
AU - Lazzarin, Adriano
AU - Meroni, Pier Luigi
AU - Panerai, Alberto E.
AU - Zanussi, Carlo
PY - 1994
Y1 - 1994
N2 - We investigated β-endorphin (BE) content in an HIV-infected cell line and in peripheral blood mononuclear cells (PBM) from HIV-positive subjects. HIV infection increased BE content in HuT78 cell line compared to uninfected cells. Accordingly, BE content was greater in HIV-positive subjects than in healthy controls, both in fresh PBM and in mitogen-stimulated or unstimulated cultured cells. Further, in PHA-stimulated cultures, BE increase was correlated with disease progression. Opioids are known to decrease immune responsiveness in vivo, and it may be that the increased BE concentrations contribute to HIV-associated immune deficiency. In HIV-positive subjects, but not in healthy controls, intracellular BE concentration was positively correlated with PHA-induced PBM proliferation. The latter data suggest an alternative explanation: that the increased BE content represents a paradoxical response of the host in an attempt to balance virus-induced immunodepression. Thus, BE may be important in fine-tuning of the immune response with its up- and downregulation dependent upon differences in immune status.
AB - We investigated β-endorphin (BE) content in an HIV-infected cell line and in peripheral blood mononuclear cells (PBM) from HIV-positive subjects. HIV infection increased BE content in HuT78 cell line compared to uninfected cells. Accordingly, BE content was greater in HIV-positive subjects than in healthy controls, both in fresh PBM and in mitogen-stimulated or unstimulated cultured cells. Further, in PHA-stimulated cultures, BE increase was correlated with disease progression. Opioids are known to decrease immune responsiveness in vivo, and it may be that the increased BE concentrations contribute to HIV-associated immune deficiency. In HIV-positive subjects, but not in healthy controls, intracellular BE concentration was positively correlated with PHA-induced PBM proliferation. The latter data suggest an alternative explanation: that the increased BE content represents a paradoxical response of the host in an attempt to balance virus-induced immunodepression. Thus, BE may be important in fine-tuning of the immune response with its up- and downregulation dependent upon differences in immune status.
KW - Cytokines
KW - HIV infection
KW - Opioids
KW - β-Endorphin
UR - http://www.scopus.com/inward/record.url?scp=0028169926&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028169926&partnerID=8YFLogxK
U2 - 10.1016/0196-9781(94)90028-0
DO - 10.1016/0196-9781(94)90028-0
M3 - Article
C2 - 7984493
AN - SCOPUS:0028169926
VL - 15
SP - 769
EP - 775
JO - Peptides
JF - Peptides
SN - 0196-9781
IS - 5
ER -