β-Nerve growth factor (β-NGF) mRNA expression in the Parkinsonian adrenal gland

V. Silani, A. Pizzuti, A. Falini, G. Borsani, E. I. Rugarli, C. A. Melo, A. Sidoli, F. Villani, F. Baralle, G. Scarlato

Research output: Contribution to journalArticle

Abstract

The adrenal gland is a well-demonstrated source for different neurotrophic factors. The presence of the β-nerve growth factor (β-NGF) mRNA in the adrenal tissue used for grafting in a Parkinsonian patient is reported here. Adrenal samples were obtained on the day of implantation, and a specific cDNA was synthesized after the extraction of total RNA using a synthetic oligonucleotide as a reverse transcription primer. A 168-bp portion of the cDNA was amplified using two other oligonucleotides as Taq polymerase primers in a polymerase chain reaction. Thirty-two cycles of amplification were performed. The amplification products were identified by agarose gel electrophoresis and Southern blot analysis as a single DNA band hybridizing with a third β-NGF specific oligonucleotide. The identity of the fragment was confirmed by DNA sequencing. Quantitative analysis demonstrated a β-NGF mRNA concentration exceeding 5 fg/μg of total adrenal RNA. These findings add NGF to the other neurotrophic factors produced by the gland (i.e., basic fibroblast growth factor) and demonstrate the retained functional capacity of the Parkinsonian adrenal to express the β-NGF mRNA. All these data may assume relevant meaning for neurotransplantation research.

Original languageEnglish
Pages (from-to)166-170
Number of pages5
JournalExperimental Neurology
Volume113
Issue number2
DOIs
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Neuroscience(all)
  • Neurology

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    Silani, V., Pizzuti, A., Falini, A., Borsani, G., Rugarli, E. I., Melo, C. A., Sidoli, A., Villani, F., Baralle, F., & Scarlato, G. (1991). β-Nerve growth factor (β-NGF) mRNA expression in the Parkinsonian adrenal gland. Experimental Neurology, 113(2), 166-170. https://doi.org/10.1016/0014-4886(91)90172-9