β2-μ-free HLA class I heavy chain levels in sera of healthy individuals. Lack of association with β2-μ-associated HLA class I heavy chain levels and HLA phenotype

F. Puppo, D. Bignardi, P. Contini, C. V. Hamby, S. Brenci, L. Lanza, M. Ghio, M. Scudeletti, F. Indiveri, Soldano Ferrone

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We have applied a double-determinant immune assay (DDIA) to measure soluble β2-microglobulin (β2-μ)-free HLA class I heavy chains in serum. The mean concentration of β2-μ-free HLA class I heavy chains in serum from 120 healthy subjects was 0.21 ± 0.24 μg/ml. The individual serum levels of β2-μ-free HLA class I heavy chains had a wide distribution, did not seem to be related with HLA phenotype, were stable over time and did not change with age. The serum levels of soluble β2-μ-free HLA class I heavy chains did not correlate with those of soluble β2-μ-associated HLA class I heavy chains, suggesting that their release is independently regulated. Three forms of soluble β2-μ-free HLA class I heavy chains, with apparent molecular masses of 44, 39 and 37-35 kD, respectively, circulate in human serum. These results provide a useful background to assess the serum level of soluble β2-μ-free HLA class I heavy chains in pathological conditions and to evaluate their putative immunoregulatory function.

Original languageEnglish
Pages (from-to)253-262
Number of pages10
JournalTissue Antigens
Volume53
Issue number3
DOIs
Publication statusPublished - 1999

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Molecular mass
Assays
Association reactions
Phenotype
Serum
Healthy Volunteers

Keywords

  • β-μ-free HLA class I heavy chains
  • β-microglobulin

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

β2-μ-free HLA class I heavy chain levels in sera of healthy individuals. Lack of association with β2-μ-associated HLA class I heavy chain levels and HLA phenotype. / Puppo, F.; Bignardi, D.; Contini, P.; Hamby, C. V.; Brenci, S.; Lanza, L.; Ghio, M.; Scudeletti, M.; Indiveri, F.; Ferrone, Soldano.

In: Tissue Antigens, Vol. 53, No. 3, 1999, p. 253-262.

Research output: Contribution to journalArticle

Puppo, F, Bignardi, D, Contini, P, Hamby, CV, Brenci, S, Lanza, L, Ghio, M, Scudeletti, M, Indiveri, F & Ferrone, S 1999, 'β2-μ-free HLA class I heavy chain levels in sera of healthy individuals. Lack of association with β2-μ-associated HLA class I heavy chain levels and HLA phenotype', Tissue Antigens, vol. 53, no. 3, pp. 253-262. https://doi.org/10.1034/j.1399-0039.1999.530305.x
Puppo, F. ; Bignardi, D. ; Contini, P. ; Hamby, C. V. ; Brenci, S. ; Lanza, L. ; Ghio, M. ; Scudeletti, M. ; Indiveri, F. ; Ferrone, Soldano. / β2-μ-free HLA class I heavy chain levels in sera of healthy individuals. Lack of association with β2-μ-associated HLA class I heavy chain levels and HLA phenotype. In: Tissue Antigens. 1999 ; Vol. 53, No. 3. pp. 253-262.
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