β2-μ-free HLA class I heavy chain levels in sera of healthy individuals. Lack of association with β2-μ-associated HLA class I heavy chain levels and HLA phenotype

F. Puppo, D. Bignardi, P. Contini, C. V. Hamby, S. Brenci, L. Lanza, M. Ghio, M. Scudeletti, F. Indiveri, Soldano Ferrone

Research output: Contribution to journalArticle

Abstract

We have applied a double-determinant immune assay (DDIA) to measure soluble β2-microglobulin (β2-μ)-free HLA class I heavy chains in serum. The mean concentration of β2-μ-free HLA class I heavy chains in serum from 120 healthy subjects was 0.21 ± 0.24 μg/ml. The individual serum levels of β2-μ-free HLA class I heavy chains had a wide distribution, did not seem to be related with HLA phenotype, were stable over time and did not change with age. The serum levels of soluble β2-μ-free HLA class I heavy chains did not correlate with those of soluble β2-μ-associated HLA class I heavy chains, suggesting that their release is independently regulated. Three forms of soluble β2-μ-free HLA class I heavy chains, with apparent molecular masses of 44, 39 and 37-35 kD, respectively, circulate in human serum. These results provide a useful background to assess the serum level of soluble β2-μ-free HLA class I heavy chains in pathological conditions and to evaluate their putative immunoregulatory function.

Original languageEnglish
Pages (from-to)253-262
Number of pages10
JournalTissue Antigens
Volume53
Issue number3
DOIs
Publication statusPublished - 1999

Keywords

  • β-μ-free HLA class I heavy chains
  • β-microglobulin

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Fingerprint Dive into the research topics of 'β2-μ-free HLA class I heavy chain levels in sera of healthy individuals. Lack of association with β2-μ-associated HLA class I heavy chain levels and HLA phenotype'. Together they form a unique fingerprint.

Cite this