β2-Glycoprotein I as a 'cofactor' for anti-phospholipid reactivity with endothelial cells

P. L. Meroni, N. Del Papa, E. Raschi, P. Panzeri, M. O. Borghi, A. Tincani, G. Balestrieri, Ma Khamashta, G. R V Hughes, T. Koike, S. A. Krilis

Research output: Contribution to journalArticle

Abstract

β2-glycoprotein I (β2GPI) is a cofactor for anti-phospholipid (aPL) binding to cardiolipin (CL)-coated plates. β2GPI is also able to bind to endothelial cell (EC) membranes as supported by in-vivo as well as by in-vitro studies. The PL-binding site in the fifth domain of the molecule is involved in the adhesion to endothelium. Actually, specific mutations in this molecular portion abolish endothelium binding and a synthetic peptide spanning the sequence Glu274-Cys288 of the CL-binding site displays comparable adhesion to EC monolayers. Heparan sulphate appears to be one of the anionic EC membrane structures with which cationic β2GPI interacts, as supported by studies with heparitinase-treated EC. β2GPI binding to EC might be related to its activity as endothelial growth factor or as a lipid-carrying glycoprotein. Adhesion of β2GPI to endothelial membranes offers suitable epitopes for circulating aPL that, once bound, can induce cell activation.

Original languageEnglish
JournalLupus
Volume7
Issue numberSUPPL. 2
Publication statusPublished - 1998

Keywords

  • β2-glycoprotein I
  • Anti-phospholipid antibodies
  • Endothelial cells
  • Heparan sulphate

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

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    Meroni, P. L., Del Papa, N., Raschi, E., Panzeri, P., Borghi, M. O., Tincani, A., Balestrieri, G., Khamashta, M., Hughes, G. R. V., Koike, T., & Krilis, S. A. (1998). β2-Glycoprotein I as a 'cofactor' for anti-phospholipid reactivity with endothelial cells. Lupus, 7(SUPPL. 2).