β1-integrins on melanoma clones regulate the interaction with autologous cytolytic T-cell clones

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Abstract

Tumor clones isolated from the same subcutaneous metastatic lesion of a melanoma patient were used to investigate the potential role of β1- integrins (VLA) in the lysability of neoplastic cells by autologous CD3+, WT31+, CD8+ cytotoxic T-cell clones. Phenotypic analysis of melanoma clones for expression of VLA molecules revealed a subset of clones with high expression of VLA-2, VLA-5, and VLA-6. This subset was also characterized by increased susceptibility to lysis by tumor-specific and nonspecific cytotoxic T-lymphocytes from either TILs or PBLs. Blocking assays with monoclonal antibodies indicated that anti-VLA-2, -5, and -6 antibodies could significantly reduce the lysis of VLA-2+, VLA-5+, and VLA-6+ melanoma clones by either specific and nonspecific CTLs. By contrast, no inhibition was seen on lysis of VLA-2-, VLA-5-, and VLA-6-negative tumor cells. These data indicate that expression of some β1-integrins on human melanoma can influence the specific and nonspecific T-cell-mediated recognition of neoplastic cells.

Original languageEnglish
Pages (from-to)183-186
Number of pages4
JournalJournal of Immunotherapy
Volume12
Issue number3
Publication statusPublished - 1992

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Keywords

  • Cytotoxic T-lymphocyte
  • Integrins
  • Lysis
  • Melanoma
  • β<inf>1</inf>-Integrins

ASJC Scopus subject areas

  • Cancer Research
  • Immunology
  • Immunology and Allergy
  • Pharmacology

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