γ δ T cells and their subpopulations in blood and synovial fluid from rheumatoid arthritis and spondyloarthritis

R. Meliconi, C. Pitzalis, G. H. Kingsley, G. S. Panayi

Research output: Contribution to journalArticle

Abstract

T cell receptor (TCR) γ δ bearing lymphocytes in peripheral blood and synovial fluid from rheumatoid arthritis (RA) and seronegative spondyloarthritides (SSA) were evaluated by means of double label immunofluorescence and cytofluorographic analysis. Three monoclonal antibodies (MAb) were used. TCR δ-1 against a common δ chain epitope, BB3 directed against the T cell subset whose TCR is encoded by the Vδ2 gene, and A13 directed against the Vδ1 subset. Peripheral blood Tγδ cells were significantly reduced compared to normal control blood in RA patients who had joint effusion but not in other RA patients. The decrease of Tγδ in the RA PB was mainly confined to the A13+ subset. In RA synovial fluid (RA SF) Tγδ cells were significantly increased compared to paired but not normal peripheral blood, the most significant being the increase of A13+ cells. In contrast, in patients with SSA, no change in Tγδ cells was observed on PB or SF. These data suggest that in RA, but not SSA, Tγδ cells migrate from PB to inflamed synovium and thus may be involved in the pathogenesis of RA.

Original languageEnglish
Pages (from-to)165-172
Number of pages8
JournalClinical Immunology and Immunopathology
Volume59
Issue number1
DOIs
Publication statusPublished - 1991

Fingerprint

Synovial Fluid
Rheumatoid Arthritis
T-Lymphocytes
T-Cell Antigen Receptor
Synovial Membrane
T-Lymphocyte Subsets
Fluorescent Antibody Technique
Epitopes
Blood Cells
Joints
Monoclonal Antibodies
Lymphocytes
Genes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

Cite this

γ δ T cells and their subpopulations in blood and synovial fluid from rheumatoid arthritis and spondyloarthritis. / Meliconi, R.; Pitzalis, C.; Kingsley, G. H.; Panayi, G. S.

In: Clinical Immunology and Immunopathology, Vol. 59, No. 1, 1991, p. 165-172.

Research output: Contribution to journalArticle

@article{69762426b109493f9954ed54a2c4f37c,
title = "γ δ T cells and their subpopulations in blood and synovial fluid from rheumatoid arthritis and spondyloarthritis",
abstract = "T cell receptor (TCR) γ δ bearing lymphocytes in peripheral blood and synovial fluid from rheumatoid arthritis (RA) and seronegative spondyloarthritides (SSA) were evaluated by means of double label immunofluorescence and cytofluorographic analysis. Three monoclonal antibodies (MAb) were used. TCR δ-1 against a common δ chain epitope, BB3 directed against the T cell subset whose TCR is encoded by the Vδ2 gene, and A13 directed against the Vδ1 subset. Peripheral blood Tγδ cells were significantly reduced compared to normal control blood in RA patients who had joint effusion but not in other RA patients. The decrease of Tγδ in the RA PB was mainly confined to the A13+ subset. In RA synovial fluid (RA SF) Tγδ cells were significantly increased compared to paired but not normal peripheral blood, the most significant being the increase of A13+ cells. In contrast, in patients with SSA, no change in Tγδ cells was observed on PB or SF. These data suggest that in RA, but not SSA, Tγδ cells migrate from PB to inflamed synovium and thus may be involved in the pathogenesis of RA.",
author = "R. Meliconi and C. Pitzalis and Kingsley, {G. H.} and Panayi, {G. S.}",
year = "1991",
doi = "10.1016/0090-1229(91)90090-W",
language = "English",
volume = "59",
pages = "165--172",
journal = "Clinical Immunology and Immunopathology",
issn = "0090-1229",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - γ δ T cells and their subpopulations in blood and synovial fluid from rheumatoid arthritis and spondyloarthritis

AU - Meliconi, R.

AU - Pitzalis, C.

AU - Kingsley, G. H.

AU - Panayi, G. S.

PY - 1991

Y1 - 1991

N2 - T cell receptor (TCR) γ δ bearing lymphocytes in peripheral blood and synovial fluid from rheumatoid arthritis (RA) and seronegative spondyloarthritides (SSA) were evaluated by means of double label immunofluorescence and cytofluorographic analysis. Three monoclonal antibodies (MAb) were used. TCR δ-1 against a common δ chain epitope, BB3 directed against the T cell subset whose TCR is encoded by the Vδ2 gene, and A13 directed against the Vδ1 subset. Peripheral blood Tγδ cells were significantly reduced compared to normal control blood in RA patients who had joint effusion but not in other RA patients. The decrease of Tγδ in the RA PB was mainly confined to the A13+ subset. In RA synovial fluid (RA SF) Tγδ cells were significantly increased compared to paired but not normal peripheral blood, the most significant being the increase of A13+ cells. In contrast, in patients with SSA, no change in Tγδ cells was observed on PB or SF. These data suggest that in RA, but not SSA, Tγδ cells migrate from PB to inflamed synovium and thus may be involved in the pathogenesis of RA.

AB - T cell receptor (TCR) γ δ bearing lymphocytes in peripheral blood and synovial fluid from rheumatoid arthritis (RA) and seronegative spondyloarthritides (SSA) were evaluated by means of double label immunofluorescence and cytofluorographic analysis. Three monoclonal antibodies (MAb) were used. TCR δ-1 against a common δ chain epitope, BB3 directed against the T cell subset whose TCR is encoded by the Vδ2 gene, and A13 directed against the Vδ1 subset. Peripheral blood Tγδ cells were significantly reduced compared to normal control blood in RA patients who had joint effusion but not in other RA patients. The decrease of Tγδ in the RA PB was mainly confined to the A13+ subset. In RA synovial fluid (RA SF) Tγδ cells were significantly increased compared to paired but not normal peripheral blood, the most significant being the increase of A13+ cells. In contrast, in patients with SSA, no change in Tγδ cells was observed on PB or SF. These data suggest that in RA, but not SSA, Tγδ cells migrate from PB to inflamed synovium and thus may be involved in the pathogenesis of RA.

UR - http://www.scopus.com/inward/record.url?scp=0025733221&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025733221&partnerID=8YFLogxK

U2 - 10.1016/0090-1229(91)90090-W

DO - 10.1016/0090-1229(91)90090-W

M3 - Article

C2 - 1826866

AN - SCOPUS:0025733221

VL - 59

SP - 165

EP - 172

JO - Clinical Immunology and Immunopathology

JF - Clinical Immunology and Immunopathology

SN - 0090-1229

IS - 1

ER -