γ-Interferon and retinoic acid synergize in inhibiting the growth of human neuroblastoma cells in nude mice

Paolo Cornaglia-Ferraris, Gian Luigi Mariottini, Mirco Ponzoni

Research output: Contribution to journalArticle

Abstract

We have investigated the effects of retinoic acid (RA), human recombinant gamma interferon (γ-IFN), and the association of both agents on the growth of human neuroblastoma (NB) cells in [CD1( nu nu)] nude mice. Two human NB cell lines, namely LAN-5 and GI-LI-N, were previously adapted to grow in syngeneic animals for 7 consecutive passages. At the eighth passage, only animals which developed 10-mm diameter tumors within 40 days from xenograft were admitted to the study. RA and/or γ-IFN were administered subcutaneously 3-5 days per week for 3 consecutive weeks. The number of days necessary for each tumor mass to grow up to 20 mm diameter (in vivo doubling time, ivDT) was then evaluated. Tumor growth was significantly inhibited in γ-IFN (P <0.005) and RA (P <0.05) treated mice grafted with GI-LI-N. The combination of the two agents did not further enhance ivDT. The tumor growth inhibition was not statistically significant in LAN-5 bearing mice treated with RA or γ-IFN alone, while a synergistic effect between the two drugs was observed (P <0.05). We conclude that parenteral combined administration of RA and γ-IFN may prove to be useful in inhibiting the growth of tumors derived from human NB cells resistant to single inducers.

Original languageEnglish
Pages (from-to)215-220
Number of pages6
JournalCancer Letters
Volume61
Issue number3
DOIs
Publication statusPublished - Jan 31 1992

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Keywords

  • neuroblastoma cells
  • nude mice
  • retinoic acid
  • γ-interferon

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

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