Abstract
The phytocannabinoid Δ9-Tetrahydrocannabinol (Δ9-THC), the main psychoactive cannabinoid in cannabis, activates a number of signalling cascades including p53. This study examines the role of Δ9-THC in regulating the p53 post-translational modifier proteins, Murine double minute (Mdm2) and Small Ubquitin-like MOdifier protein 1 (SUMO-1) in cortical neurons. Δ9-THC increased both Mdm2 and SUMO-1 protein expression and induced the deSUMOylation of p53 in a cannabinoid receptor type 1 (CB1)-receptor dependent manner. We demonstrate that Δ9-THC decreased the SUMOylation of the CB1 receptor. The data reveal a novel role for cannabinoid receptor activation in modulating the SUMO regulatory system. Structured summary: MINT-7266621: Cb1 (uniprotkb:P20272) physically interacts (MI:0915) with SUMO-1 (uniprotkb:Q5I0H3) by anti bait coimmunoprecipitation. (MI:0006)MINT-7266633: SUMO-1 (uniprotkb:Q5I0H3) and Cb1 (uniprotkb:P20272) colocalize (MI:0403) by fluorescence microscopy (MI:0416). MINT-7266611: p53 (uniprotkb:P10361) physically interacts (MI:0915) with SUMO-1 (uniprotkb:Q5I0H3) by anti bait coimmunoprecipitation (MI:0006).
Original language | English |
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Pages (from-to) | 3412-3418 |
Number of pages | 7 |
Journal | FEBS Letters |
Volume | 583 |
Issue number | 21 |
DOIs | |
Publication status | Published - Nov 3 2009 |
Keywords
- Δ-Tetrahydrocannabinol
- Cannabinoid
- Murine double minute
- Post-translational modification
- Signal transduction
- Small Ubquitin-like MOdifier
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
- Genetics
- Molecular Biology
- Structural Biology