1α,25-Dihydroxyvitamin D3 inhibits CD40L-induced pro-inflammatory and immunomodulatory activity in Human Monocytes

Cristiana Almerighi, Anna Sinistro, Antonella Cavazza, Chiara Ciaprini, Giovanni Rocchi, Alberto Bergamini

Research output: Contribution to journalArticlepeer-review

Abstract

CD40 ligand (CD40L) stimulation induces proinflammatory and immunomodulatory activity in monocytes. Here, we report on the effects of the steroid hormone 1α,25-dihydroxyvitamin D3 (1,25D3) on human blood monocytes that have been stimulated with the CD40L ligand. Co-treatment of CD40L-stimulated monocytes with 1,25D3 resulted in reduced production and secretion of tumor necrosis factor (TNF)-α and interleukin (IL)-1β, as well as in reduced expression of the surface co-stimulatory molecules CD80 and CD86. In addition, costimulation of CD4+ T lymphocytes by monocytes co-treated with CD40L and 1,25D3 resulted in reduced cell proliferation and diminished interferon (IFN)-γ but enhanced IL-10 production by CD4+ T cells. Finally, 1,25D3 interfered with the ability of CD40L to rescue monocytes from apoptosis induced by serum withdrawal. These findings suggest that 1,25D3 may regulate the interaction of monocytes with T cells or other cell types that express CD40L, thus influencing the outcome of the immune or inflammatory response.

Original languageEnglish
Pages (from-to)190-197
Number of pages8
JournalCytokine
Volume45
Issue number3
DOIs
Publication statusPublished - Mar 2009

Keywords

  • 1α,25-Dihydroxyvitamin D
  • CD40L
  • Cytokine
  • Monocyte
  • T cell

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Immunology
  • Hematology
  • Immunology and Allergy

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