Abstract
The modulation of CD44, VCAM-1 and CD71 expression was analysed by flow cytometry in the 1321N1 astrocytoma cell line in the presence of interleukin-1β (IL1β), tumour necrosis factor-α (TNFα) and 1-40 or 25-35 β-amyloid (Aβ) fragments. The percentage of 1321N1 astrocytoma cell line expressing these markers increased significantly after treatment with TNFα or IL1β. The presence of Aβ 1-40 fragment, alone or in combination with IL1β, induced an increase in the percentage of cells expressing CD44, but not VCAM-1. However, the concomitant presence of Aβ 1-40 fragment and of IL1β or TNFα caused an increase in the percentage of CD71 positive cells. In contrast, the shorter Aβ 25-35 fragment was always inactive. These results indicates that Aβ 1-40 fragment, in association with cytokines, can activate this astrocyte-derived cell line and add further elements in favour of the hypothesis that β-amyloid can act as immunological mediator.
| Original language | English |
|---|---|
| Pages (from-to) | 190-195 |
| Number of pages | 6 |
| Journal | Journal of Cellular Physiology |
| Volume | 196 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jul 1 2003 |
Fingerprint
ASJC Scopus subject areas
- Clinical Biochemistry
- Cell Biology
- Physiology
Cite this
1-40 β-amyloid protein fragment modulates the expression of CD44 and CD71 on the astrocytoma cell line in the presence of IL1β and TNFα. / Speciale, Livianna; Ruzzante, Stefania; Calabrese, Elena; Saresella, Marina; Taramelli, Donatella; Mariani, Claudio; Bava, Laura; Longhi, Renato; Ferrante, Pasquale.
In: Journal of Cellular Physiology, Vol. 196, No. 1, 01.07.2003, p. 190-195.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - 1-40 β-amyloid protein fragment modulates the expression of CD44 and CD71 on the astrocytoma cell line in the presence of IL1β and TNFα
AU - Speciale, Livianna
AU - Ruzzante, Stefania
AU - Calabrese, Elena
AU - Saresella, Marina
AU - Taramelli, Donatella
AU - Mariani, Claudio
AU - Bava, Laura
AU - Longhi, Renato
AU - Ferrante, Pasquale
PY - 2003/7/1
Y1 - 2003/7/1
N2 - The modulation of CD44, VCAM-1 and CD71 expression was analysed by flow cytometry in the 1321N1 astrocytoma cell line in the presence of interleukin-1β (IL1β), tumour necrosis factor-α (TNFα) and 1-40 or 25-35 β-amyloid (Aβ) fragments. The percentage of 1321N1 astrocytoma cell line expressing these markers increased significantly after treatment with TNFα or IL1β. The presence of Aβ 1-40 fragment, alone or in combination with IL1β, induced an increase in the percentage of cells expressing CD44, but not VCAM-1. However, the concomitant presence of Aβ 1-40 fragment and of IL1β or TNFα caused an increase in the percentage of CD71 positive cells. In contrast, the shorter Aβ 25-35 fragment was always inactive. These results indicates that Aβ 1-40 fragment, in association with cytokines, can activate this astrocyte-derived cell line and add further elements in favour of the hypothesis that β-amyloid can act as immunological mediator.
AB - The modulation of CD44, VCAM-1 and CD71 expression was analysed by flow cytometry in the 1321N1 astrocytoma cell line in the presence of interleukin-1β (IL1β), tumour necrosis factor-α (TNFα) and 1-40 or 25-35 β-amyloid (Aβ) fragments. The percentage of 1321N1 astrocytoma cell line expressing these markers increased significantly after treatment with TNFα or IL1β. The presence of Aβ 1-40 fragment, alone or in combination with IL1β, induced an increase in the percentage of cells expressing CD44, but not VCAM-1. However, the concomitant presence of Aβ 1-40 fragment and of IL1β or TNFα caused an increase in the percentage of CD71 positive cells. In contrast, the shorter Aβ 25-35 fragment was always inactive. These results indicates that Aβ 1-40 fragment, in association with cytokines, can activate this astrocyte-derived cell line and add further elements in favour of the hypothesis that β-amyloid can act as immunological mediator.
UR - http://www.scopus.com/inward/record.url?scp=0037945813&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037945813&partnerID=8YFLogxK
U2 - 10.1002/jcp.10295
DO - 10.1002/jcp.10295
M3 - Article
C2 - 12767055
AN - SCOPUS:0037945813
VL - 196
SP - 190
EP - 195
JO - Journal of cellular and comparative physiology
JF - Journal of cellular and comparative physiology
SN - 0021-9541
IS - 1
ER -