[11C] Methylation on a C18 Sep-Pak cartridge: A convenient way to produce [N-methyl-11C] choline

Claudio Pascali, Anna Bogni, Ren Iwata, Mara Cambiè, Emilio Bombardieri

Research output: Contribution to journalArticlepeer-review


[N-Methyl-11C]choline has been simply and efficiently labelled at room temperature by solid supported [11C]methylation of 2-dimethylaminoethanol on a commercial C18 Sep-Pak Light cartridge. The labelled product was retained on a cation exchange resin (Sep-Pak Plus Accell Plus CM) and washed with ethanol and water to remove any excess precursor. Then [N-methyl-11C]choline was desorbed from the cartridge by elution with saline, passed through a 0.2 mm sterile filter and collected in a sterile vial. Overall, the total synthesis time was 12 min from the end of bombardment (EOB). The radiochemical yield from [11C]CO2, decay corrected (EOB) was 87 and the radiochemical purity was greater than 99. Very conveniently, the automated apparatus employed is a very simple modification of the system routinely used for the synthesis of L-[S-methyl-11C]methionine.

Original languageEnglish
Pages (from-to)195-203
Number of pages9
JournalJournal of Labelled Compounds and Radiopharmaceuticals
Issue number2
Publication statusPublished - 2000


  • [C] choline
  • PET
  • Solid supported methylation

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Medicine
  • Analytical Chemistry
  • Organic Chemistry
  • Drug Discovery
  • Pharmacology


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