Abstract
[N-Methyl-11C]choline has been simply and efficiently labelled at room temperature by solid supported [11C]methylation of 2-dimethylaminoethanol on a commercial C18 Sep-Pak Light cartridge. The labelled product was retained on a cation exchange resin (Sep-Pak Plus Accell Plus CM) and washed with ethanol and water to remove any excess precursor. Then [N-methyl-11C]choline was desorbed from the cartridge by elution with saline, passed through a 0.2 mm sterile filter and collected in a sterile vial. Overall, the total synthesis time was 12 min from the end of bombardment (EOB). The radiochemical yield from [11C]CO2, decay corrected (EOB) was 87 and the radiochemical purity was greater than 99. Very conveniently, the automated apparatus employed is a very simple modification of the system routinely used for the synthesis of L-[S-methyl-11C]methionine.
Original language | English |
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Pages (from-to) | 195-203 |
Number of pages | 9 |
Journal | Journal of Labelled Compounds and Radiopharmaceuticals |
Volume | 43 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2000 |
Keywords
- [C] choline
- PET
- Solid supported methylation
ASJC Scopus subject areas
- Clinical Biochemistry
- Molecular Medicine
- Analytical Chemistry
- Organic Chemistry
- Drug Discovery
- Pharmacology