1,2-Dichloropropane hepatotoxicity in rats after inhalation exposure

A. Di Nucci, C. Gregotti, L. Manzo, M. Imbriani, S. Ghittori, L. Bianco, L. Maestri, E. Capodaglio

Research output: Contribution to journalArticle

Abstract

The hepatic effects of 1,2-dichloropropane (DCP) were investigated in male Wistar rats exposed to 15, 50, 100, 250, 450, 1000, 1300, 1800 or 4900 mg DCP m-3. At the end of a 4-h period of exposure, average blood DCP levels were 0.025 and 5.38 μg ml-1 in animals treated with 15 and 1300 mg m-3, respectively. Blood DCP concentrations were correlated with the air DCP concentrations in the inhalation chamber. At DCP concentrations of 100 mg m-3 or higher, the liver non-protein thiol (NPT) content was significantly reduced. Assays performed 20 h after 4-h DCP exposure showed that exposure to 100-1000 mg DCP m-3 had no effect on hepatic NPT levels. The NPT content increased only in the liver of rats exposed to higher (1300-4900 mg m-3) DCP concentrations. Treatment with DCP did not cause hepatic lipid peroxidation and did not modify total protein content. The observed changes in liver cell thiol homeostasis are likely to reflect the action of reactive intermediates formed during DCP metabolism. These changes can occur in rats following exposure to considerably low levels of DCP vapour.

Original languageEnglish
Pages (from-to)391-394
Number of pages4
JournalJournal of Applied Toxicology
Volume10
Issue number6
Publication statusPublished - 1990

Fingerprint

Inhalation Exposure
Rats
Sulfhydryl Compounds
Liver
propylene dichloride
Blood
Metabolism
Inhalation
Lipid Peroxidation
Wistar Rats
Assays
Animals
Homeostasis

Keywords

  • 1,2-dichloropropane
  • gas chromatography
  • glutathione
  • halocarbons
  • head-space technique
  • hepatotoxicity
  • inhalation exposure
  • solvents

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Di Nucci, A., Gregotti, C., Manzo, L., Imbriani, M., Ghittori, S., Bianco, L., ... Capodaglio, E. (1990). 1,2-Dichloropropane hepatotoxicity in rats after inhalation exposure. Journal of Applied Toxicology, 10(6), 391-394.

1,2-Dichloropropane hepatotoxicity in rats after inhalation exposure. / Di Nucci, A.; Gregotti, C.; Manzo, L.; Imbriani, M.; Ghittori, S.; Bianco, L.; Maestri, L.; Capodaglio, E.

In: Journal of Applied Toxicology, Vol. 10, No. 6, 1990, p. 391-394.

Research output: Contribution to journalArticle

Di Nucci, A, Gregotti, C, Manzo, L, Imbriani, M, Ghittori, S, Bianco, L, Maestri, L & Capodaglio, E 1990, '1,2-Dichloropropane hepatotoxicity in rats after inhalation exposure', Journal of Applied Toxicology, vol. 10, no. 6, pp. 391-394.
Di Nucci A, Gregotti C, Manzo L, Imbriani M, Ghittori S, Bianco L et al. 1,2-Dichloropropane hepatotoxicity in rats after inhalation exposure. Journal of Applied Toxicology. 1990;10(6):391-394.
Di Nucci, A. ; Gregotti, C. ; Manzo, L. ; Imbriani, M. ; Ghittori, S. ; Bianco, L. ; Maestri, L. ; Capodaglio, E. / 1,2-Dichloropropane hepatotoxicity in rats after inhalation exposure. In: Journal of Applied Toxicology. 1990 ; Vol. 10, No. 6. pp. 391-394.
@article{3ea41747594645daa74cec99c1620749,
title = "1,2-Dichloropropane hepatotoxicity in rats after inhalation exposure",
abstract = "The hepatic effects of 1,2-dichloropropane (DCP) were investigated in male Wistar rats exposed to 15, 50, 100, 250, 450, 1000, 1300, 1800 or 4900 mg DCP m-3. At the end of a 4-h period of exposure, average blood DCP levels were 0.025 and 5.38 μg ml-1 in animals treated with 15 and 1300 mg m-3, respectively. Blood DCP concentrations were correlated with the air DCP concentrations in the inhalation chamber. At DCP concentrations of 100 mg m-3 or higher, the liver non-protein thiol (NPT) content was significantly reduced. Assays performed 20 h after 4-h DCP exposure showed that exposure to 100-1000 mg DCP m-3 had no effect on hepatic NPT levels. The NPT content increased only in the liver of rats exposed to higher (1300-4900 mg m-3) DCP concentrations. Treatment with DCP did not cause hepatic lipid peroxidation and did not modify total protein content. The observed changes in liver cell thiol homeostasis are likely to reflect the action of reactive intermediates formed during DCP metabolism. These changes can occur in rats following exposure to considerably low levels of DCP vapour.",
keywords = "1,2-dichloropropane, gas chromatography, glutathione, halocarbons, head-space technique, hepatotoxicity, inhalation exposure, solvents",
author = "{Di Nucci}, A. and C. Gregotti and L. Manzo and M. Imbriani and S. Ghittori and L. Bianco and L. Maestri and E. Capodaglio",
year = "1990",
language = "English",
volume = "10",
pages = "391--394",
journal = "Journal of Applied Toxicology",
issn = "0260-437X",
publisher = "John Wiley and Sons Ltd",
number = "6",

}

TY - JOUR

T1 - 1,2-Dichloropropane hepatotoxicity in rats after inhalation exposure

AU - Di Nucci, A.

AU - Gregotti, C.

AU - Manzo, L.

AU - Imbriani, M.

AU - Ghittori, S.

AU - Bianco, L.

AU - Maestri, L.

AU - Capodaglio, E.

PY - 1990

Y1 - 1990

N2 - The hepatic effects of 1,2-dichloropropane (DCP) were investigated in male Wistar rats exposed to 15, 50, 100, 250, 450, 1000, 1300, 1800 or 4900 mg DCP m-3. At the end of a 4-h period of exposure, average blood DCP levels were 0.025 and 5.38 μg ml-1 in animals treated with 15 and 1300 mg m-3, respectively. Blood DCP concentrations were correlated with the air DCP concentrations in the inhalation chamber. At DCP concentrations of 100 mg m-3 or higher, the liver non-protein thiol (NPT) content was significantly reduced. Assays performed 20 h after 4-h DCP exposure showed that exposure to 100-1000 mg DCP m-3 had no effect on hepatic NPT levels. The NPT content increased only in the liver of rats exposed to higher (1300-4900 mg m-3) DCP concentrations. Treatment with DCP did not cause hepatic lipid peroxidation and did not modify total protein content. The observed changes in liver cell thiol homeostasis are likely to reflect the action of reactive intermediates formed during DCP metabolism. These changes can occur in rats following exposure to considerably low levels of DCP vapour.

AB - The hepatic effects of 1,2-dichloropropane (DCP) were investigated in male Wistar rats exposed to 15, 50, 100, 250, 450, 1000, 1300, 1800 or 4900 mg DCP m-3. At the end of a 4-h period of exposure, average blood DCP levels were 0.025 and 5.38 μg ml-1 in animals treated with 15 and 1300 mg m-3, respectively. Blood DCP concentrations were correlated with the air DCP concentrations in the inhalation chamber. At DCP concentrations of 100 mg m-3 or higher, the liver non-protein thiol (NPT) content was significantly reduced. Assays performed 20 h after 4-h DCP exposure showed that exposure to 100-1000 mg DCP m-3 had no effect on hepatic NPT levels. The NPT content increased only in the liver of rats exposed to higher (1300-4900 mg m-3) DCP concentrations. Treatment with DCP did not cause hepatic lipid peroxidation and did not modify total protein content. The observed changes in liver cell thiol homeostasis are likely to reflect the action of reactive intermediates formed during DCP metabolism. These changes can occur in rats following exposure to considerably low levels of DCP vapour.

KW - 1,2-dichloropropane

KW - gas chromatography

KW - glutathione

KW - halocarbons

KW - head-space technique

KW - hepatotoxicity

KW - inhalation exposure

KW - solvents

UR - http://www.scopus.com/inward/record.url?scp=0025077165&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025077165&partnerID=8YFLogxK

M3 - Article

C2 - 2084176

AN - SCOPUS:0025077165

VL - 10

SP - 391

EP - 394

JO - Journal of Applied Toxicology

JF - Journal of Applied Toxicology

SN - 0260-437X

IS - 6

ER -