Tumor heterogeneity, an important characteristic of gliomas, may reflect genetic instability. Heterogeneity in astrocytic tumors has been recognized from histology, karyotype, oncogene expression and intrinsic resistance to chemotherapy and radiation therapy. In the present study, we demonstrated heterogeneity by analysis of chromosomal aberrations using the fluorescence in situ hybridization (FISH) technique. FISH permits direct visualization of gains and losses of genetic material in single cells and quantitation of cellular subpopulation that have particular genetic aberrations. DNA probes for chromosomes 1,8, 12, 17 were used. The preliminary results on 10 examined tumors showed the following percentages of aneusomic cells 55.80, 52.40, 48.60, 52.10 for chromosome 1, 8, 12, 17 respectively. These findings demonstrate that FISH technique using chromosome specific pericentromeric probes allowed the detection of aneuploidy at the level of single chromosomes. Furthermore, using probes for chromosomes already described to be frequently involved in numerical aberrations in astrocytomas, we can detect aneuploid events with higher sensitivity and increased specificity. Information about aneuploidy of these chromosomes may help us to learn more about astrocytoma oncogenesis and an analysis of a large series of patients could determine the diagnostic and prognostic relevance in the future.
|Number of pages||2|
|Journal||Italian Journal of Neurological Sciences|
|Publication status||Published - 1997|
ASJC Scopus subject areas
- Clinical Neurology