TY - JOUR
T1 - 16s rRNA of mucosal colon microbiome and CCL2 circulating levels are potential biomarkers in colorectal cancer
AU - Nardelli, Carmela
AU - Granata, Ilaria
AU - Nunziato, Marcella
AU - Setaro, Mario
AU - Carbone, Fortunata
AU - Zulli, Claudio
AU - Pilone, Vincenzo
AU - Capoluongo, Ettore Domenico
AU - De Palma, Giovanni Domenico
AU - Corcione, Francesco
AU - Matarese, Giuseppe
AU - Salvatore, Francesco
AU - Sacchetti, Lucia
N1 - Funding Information:
Funding: This research was funded by research project SATIN (Sviluppo di Approcci Terapeutici Innovativi per patologie neoplastiche resistenti ai trattamenti) D.D. n. 355 del 5/06/2017–Fondo FESR 2014/2020 to F.S. and L.S.; G.M. is funded by Fondazione Italiana Sclerosi Multipla (FISM; grants 2016/R/18 and 2018/S/5), Progetti di Rilevante Interesse Nazionale (PRIN; grant 2017K55HLC 001), and Ministero della Salute (grant RF‐2019‐12371111); F.C. is supported by the Ministero della Salute (grant GR‐2016‐02363725).
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/10/1
Y1 - 2021/10/1
N2 - Colorectal cancer (CRC) is one of the most common malignancies in the Western world and intestinal dysbiosis might contribute to its pathogenesis. The mucosal colon microbiome and C‐C motif chemokine 2 (CCL2) were investigated in 20 healthy controls (HC) and 20 CRC patients using 16S rRNA sequencing and immunoluminescent assay, respectively. A total of 10 HC subjects were classified as overweight/obese (OW/OB_HC) and 10 subjects were normal weight (NW_HC); 15 CRC patients were classified as OW/OB_CRC and 5 patients were NW_CRC. Results: Fusobacterium nucleatum and Escherichia coli were more abundant in OW/OB_HC than in NW_HC microbiomes. Globally, Streptococcus intermedius, Gemella haemolysans, Fusobacterium nucleatum, Bacteroides fragilis and Escherichia coli were significantly increased in CRC patient tumor/lesioned tissue (CRC_LT) and CRC patient unlesioned tissue (CRC_ULT) microbiomes compared to HC microbiomes. CCL2 circulating levels were associated with tumor presence and with the abundance of Fusobacterium nucleatum, Bacteroides fragilis and Gemella haemolysans. Our data suggest that mucosal colon dysbiosis might contribute to CRC pathogenesis by inducing inflammation. Notably, Fusobacterium nucleatum, which was more abundant in the OW/OB_HC than in the NW_HC microbiomes, might represent a putative link between obesity and increased CRC risk.
AB - Colorectal cancer (CRC) is one of the most common malignancies in the Western world and intestinal dysbiosis might contribute to its pathogenesis. The mucosal colon microbiome and C‐C motif chemokine 2 (CCL2) were investigated in 20 healthy controls (HC) and 20 CRC patients using 16S rRNA sequencing and immunoluminescent assay, respectively. A total of 10 HC subjects were classified as overweight/obese (OW/OB_HC) and 10 subjects were normal weight (NW_HC); 15 CRC patients were classified as OW/OB_CRC and 5 patients were NW_CRC. Results: Fusobacterium nucleatum and Escherichia coli were more abundant in OW/OB_HC than in NW_HC microbiomes. Globally, Streptococcus intermedius, Gemella haemolysans, Fusobacterium nucleatum, Bacteroides fragilis and Escherichia coli were significantly increased in CRC patient tumor/lesioned tissue (CRC_LT) and CRC patient unlesioned tissue (CRC_ULT) microbiomes compared to HC microbiomes. CCL2 circulating levels were associated with tumor presence and with the abundance of Fusobacterium nucleatum, Bacteroides fragilis and Gemella haemolysans. Our data suggest that mucosal colon dysbiosis might contribute to CRC pathogenesis by inducing inflammation. Notably, Fusobacterium nucleatum, which was more abundant in the OW/OB_HC than in the NW_HC microbiomes, might represent a putative link between obesity and increased CRC risk.
KW - 16S rRNA gene
KW - CCL2 [chemokine (C‐C motif) ligand 2]
KW - Colorectal cancer
KW - Mucosal colon microbiome
KW - Obesity
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U2 - 10.3390/ijms221910747
DO - 10.3390/ijms221910747
M3 - Article
AN - SCOPUS:85116231987
VL - 22
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 19
M1 - 10747
ER -