17β-estradiol effects on human coronaries and grafts employed in myocardial revascularization

A preliminary study

Gianluca Polvani, Fabio Barili, Giuseppe Rossoni, Luca Dainese, Manuela Wally Ossola, Veli K. Topkara, Francesco Grillo, Eleonora Penza, Elena Tremoli, Paolo Biglioli

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: This study was undertaken to compare the in vitro effects of 17β-estradiol on human epicardial coronary arteries, resistance coronary arteries and on arterial vessels usually employed as grafts in surgical myocardial revascularization. Methods: Coronary artery rings (descending coronary artery, right coronary artery, circumflex coronary artery, first septal branch) and arterial graft rings (internal thoracic artery, gastro-epiploic artery) obtained from human heart donors with heart not suitable to cardiac transplantation were connected to force transducer for isometric force recording. Precontracted specimens with and without endothelium were exposed to increasing concentration of 17β-estradiol (3-30-300-3000 nmol/l) and to vehicle (0.1% v/v ethanol). We also evaluated the effects of 17β-estradiol on vessels before and 20 minutes after exposure to L-monomethyl-arginine and indomethacin. Results: 17β-estradiol induced a significant relaxation in all precontracted vessels (mean maximum effect: 78,6% ± 8,5). This effect was not different among the different rings and was not related to the presence of endothelium. N-monomethyl-L-arginine and indomethacin did not modify 17β-estradiol relaxant effect. Conclusion: The vasodilator action of the 17β-estradiol is similar on coronary arteries, resistance coronary arteries and arterial vessels usually employed as grafts in myocardial revascularization.

Original languageEnglish
Article number46
JournalJournal of Cardiothoracic Surgery
Volume1
Issue number1
DOIs
Publication statusPublished - Dec 20 2006

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Myocardial Revascularization
Estradiol
Coronary Vessels
Transplants
Indomethacin
Endothelium
Arginine
Mammary Arteries
Heart Transplantation
Transducers
Vasodilator Agents
Ethanol
Arteries

ASJC Scopus subject areas

  • Surgery
  • Cardiology and Cardiovascular Medicine

Cite this

17β-estradiol effects on human coronaries and grafts employed in myocardial revascularization : A preliminary study. / Polvani, Gianluca; Barili, Fabio; Rossoni, Giuseppe; Dainese, Luca; Ossola, Manuela Wally; Topkara, Veli K.; Grillo, Francesco; Penza, Eleonora; Tremoli, Elena; Biglioli, Paolo.

In: Journal of Cardiothoracic Surgery, Vol. 1, No. 1, 46, 20.12.2006.

Research output: Contribution to journalArticle

Polvani, Gianluca ; Barili, Fabio ; Rossoni, Giuseppe ; Dainese, Luca ; Ossola, Manuela Wally ; Topkara, Veli K. ; Grillo, Francesco ; Penza, Eleonora ; Tremoli, Elena ; Biglioli, Paolo. / 17β-estradiol effects on human coronaries and grafts employed in myocardial revascularization : A preliminary study. In: Journal of Cardiothoracic Surgery. 2006 ; Vol. 1, No. 1.
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abstract = "Background: This study was undertaken to compare the in vitro effects of 17β-estradiol on human epicardial coronary arteries, resistance coronary arteries and on arterial vessels usually employed as grafts in surgical myocardial revascularization. Methods: Coronary artery rings (descending coronary artery, right coronary artery, circumflex coronary artery, first septal branch) and arterial graft rings (internal thoracic artery, gastro-epiploic artery) obtained from human heart donors with heart not suitable to cardiac transplantation were connected to force transducer for isometric force recording. Precontracted specimens with and without endothelium were exposed to increasing concentration of 17β-estradiol (3-30-300-3000 nmol/l) and to vehicle (0.1{\%} v/v ethanol). We also evaluated the effects of 17β-estradiol on vessels before and 20 minutes after exposure to L-monomethyl-arginine and indomethacin. Results: 17β-estradiol induced a significant relaxation in all precontracted vessels (mean maximum effect: 78,6{\%} ± 8,5). This effect was not different among the different rings and was not related to the presence of endothelium. N-monomethyl-L-arginine and indomethacin did not modify 17β-estradiol relaxant effect. Conclusion: The vasodilator action of the 17β-estradiol is similar on coronary arteries, resistance coronary arteries and arterial vessels usually employed as grafts in myocardial revascularization.",
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AU - Ossola, Manuela Wally

AU - Topkara, Veli K.

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AB - Background: This study was undertaken to compare the in vitro effects of 17β-estradiol on human epicardial coronary arteries, resistance coronary arteries and on arterial vessels usually employed as grafts in surgical myocardial revascularization. Methods: Coronary artery rings (descending coronary artery, right coronary artery, circumflex coronary artery, first septal branch) and arterial graft rings (internal thoracic artery, gastro-epiploic artery) obtained from human heart donors with heart not suitable to cardiac transplantation were connected to force transducer for isometric force recording. Precontracted specimens with and without endothelium were exposed to increasing concentration of 17β-estradiol (3-30-300-3000 nmol/l) and to vehicle (0.1% v/v ethanol). We also evaluated the effects of 17β-estradiol on vessels before and 20 minutes after exposure to L-monomethyl-arginine and indomethacin. Results: 17β-estradiol induced a significant relaxation in all precontracted vessels (mean maximum effect: 78,6% ± 8,5). This effect was not different among the different rings and was not related to the presence of endothelium. N-monomethyl-L-arginine and indomethacin did not modify 17β-estradiol relaxant effect. Conclusion: The vasodilator action of the 17β-estradiol is similar on coronary arteries, resistance coronary arteries and arterial vessels usually employed as grafts in myocardial revascularization.

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