17β-estradiol rescues spinal motoneurons from AMPA-induced toxicity: A role for glial cells

Paola Platania, Giovanna Seminara, Eleonora Aronica, Dirk Troost, Maria Vincenza Catania, Maria Angela Sortino

Research output: Contribution to journalArticlepeer-review

Abstract

The ability of astrocytes to mediate 17β-estradiol neuroprotection of spinal motoneurons challenged with AMPA has been evaluated in a co-culture system in which pure motoneurons were pulsed with 20 μM AMPA and then transferred onto an astrocyte layer pretreated for 24 h with 10 nM 17β-estradiol. Under these conditions, AMPA toxicity was reverted, an effect that was likely related to increased production and release of GDNF, as shown by RT-PCR, Western blot analysis and ELISA assay. In addition, treatment with GDNF during the 24 h that followed the AMPA pulse produced a similar neuroprotective effect, whereas addition of a neutralizing anti-GDNF antibody prevented neuroprotection. These data suggest a role for astrocytes in the neuroprotective effect of 17β-estradiol against spinal motoneuron death and find strong support in the marked up-regulation of estrogen receptor α found in spinal astrocytes of amyotrophic lateral sclerosis patients.

Original languageEnglish
Pages (from-to)461-470
Number of pages10
JournalNeurobiology of Disease
Volume20
Issue number2
DOIs
Publication statusPublished - Nov 2005

Keywords

  • Amyotrophic lateral sclerosis
  • Astrocytes
  • Glia
  • Glutamate
  • Neurodegeneration
  • Neuroprotection

ASJC Scopus subject areas

  • Neurology

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