17-β-estradiol elicits genomic and non-genomic responses in mouse male germ cells

Elena Vicini, Maria Loiarro, Silvia Di Agostino, Serena Corallini, Federica Capolunghi, Rita Carsetti, Paolo Chieffi, Raffaele Geremia, Mario Stefanini, Claudio Sette

Research output: Contribution to journalArticle

Abstract

Estrogens have been postulated to exert a detrimental effect on spermatogenesis in vivo. Since mouse male germ cells express estrogen receptors, we have investigated whether molecular pathways are activated by estrogen stimulation of these cells. Our results demonstrate that estrogen receptor β is expressed in mitotic and meiotic male germ cells as well as in the spermatogonia derived GC-1 cell line. By using this cell line, we show that 17-β-estradiol triggers activation of a transcriptional response that requires a functional estrogen receptor. Moreover, GC-1 cells respond to estrogens by transiently activating a signal transduction pathway that impinges on the mitogen-activated protein kinases (MAPK) ERK1 and -2. A similar dose-dependent transient activation of ERKs was also observed in primary mouse spermatocytes in culture. Activation by the estrogen was specific because other steroids such as progesterone and dihydrotestosterone were ineffective and because it could be blocked by the selective inhibitor of the ERK pathway and by competitive inhibitors of the estrogen receptor. Finally, we observed that 17-β-estradiol does not affect spontaneous or induced apoptosis in cultured mouse spermatocytes, indicating that the apoptotic effects observed in vivo require additional testicular components.

Original languageEnglish
Pages (from-to)238-245
Number of pages8
JournalJournal of Cellular Physiology
Volume206
Issue number1
DOIs
Publication statusPublished - Jan 2006

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Germ Cells
Estrogen Receptors
Estradiol
Estrogens
Cells
Spermatocytes
Chemical activation
Cell Line
Signal transduction
Spermatogonia
MAP Kinase Signaling System
Dihydrotestosterone
Mitogen-Activated Protein Kinase 1
Spermatogenesis
Mitogen-Activated Protein Kinases
Transcriptional Activation
Progesterone
Signal Transduction
Steroids
Apoptosis

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

17-β-estradiol elicits genomic and non-genomic responses in mouse male germ cells. / Vicini, Elena; Loiarro, Maria; Di Agostino, Silvia; Corallini, Serena; Capolunghi, Federica; Carsetti, Rita; Chieffi, Paolo; Geremia, Raffaele; Stefanini, Mario; Sette, Claudio.

In: Journal of Cellular Physiology, Vol. 206, No. 1, 01.2006, p. 238-245.

Research output: Contribution to journalArticle

Vicini, E, Loiarro, M, Di Agostino, S, Corallini, S, Capolunghi, F, Carsetti, R, Chieffi, P, Geremia, R, Stefanini, M & Sette, C 2006, '17-β-estradiol elicits genomic and non-genomic responses in mouse male germ cells', Journal of Cellular Physiology, vol. 206, no. 1, pp. 238-245. https://doi.org/10.1002/jcp.20454
Vicini, Elena ; Loiarro, Maria ; Di Agostino, Silvia ; Corallini, Serena ; Capolunghi, Federica ; Carsetti, Rita ; Chieffi, Paolo ; Geremia, Raffaele ; Stefanini, Mario ; Sette, Claudio. / 17-β-estradiol elicits genomic and non-genomic responses in mouse male germ cells. In: Journal of Cellular Physiology. 2006 ; Vol. 206, No. 1. pp. 238-245.
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