17A, a novel non-coding RNA, regulates GABA B alternative splicing and signaling in response to inflammatory stimuli and in Alzheimer disease

Sara Massone, Irene Vassallo, Gloria Fiorino, Manuele Castelnuovo, Federica Barbieri, Roberta Borghi, Massimo Tabaton, Mauro Robello, Elena Gatta, Claudio Russo, Tullio Florio, Giorgio Dieci, Ranieri Cancedda, Aldo Pagano

Research output: Contribution to journalArticle

Abstract

Alternative splicing is a central component of human brain complexity; nonetheless, its regulatory mechanisms are still largely unclear. In this work, we describe a novel non-coding (nc) RNA (named 17A) RNA polymerase (pol) III-dependent embedded in the human G-protein-coupled receptor 51 gene (GPR51, GABA B2 receptor). The stable expression of 17A in SHSY5Y neuroblastoma cells induces the synthesis of an alternative splicing isoform that abolish GABA B2 intracellular signaling (i.e., inhibition of cAMP accumulation and activation of K + channels). Indeed, 17A is expressed in human brain, and we report that it is upregulated in cerebral tissues derived from Alzheimer disease patients. We demonstrate that 17A expression in neuroblastoma cells enhances the secretion of amyloid β peptide (Aβ) and the Aβ x-42/αβ x-40 peptide ratio and that its synthesis is induced in response to inflammatory stimuli. These data correlate, for the first time, the activity of a novel pol III-dependent ncRNA to alternative splicing events and, possibly, to neurodegeneration induced by abnormal GABA B function. We anticipate that further analysis of pol III-dependent regulation of alternative splicing will disclose novel regulatory pathways associated to brain physiology and/or pathology.

Original languageEnglish
Pages (from-to)308-317
Number of pages10
JournalNeurobiology of Disease
Volume41
Issue number2
DOIs
Publication statusPublished - Feb 2011

Fingerprint

Untranslated RNA
Alternative Splicing
gamma-Aminobutyric Acid
Alzheimer Disease
Neuroblastoma
Brain
RNA Polymerase III
GABA Receptors
Amyloid
Protein Isoforms
Pathology
Peptides
Genes

Keywords

  • Amyloid β
  • Inflammation
  • Neurodegeneration
  • RNA polymerase III

ASJC Scopus subject areas

  • Neurology

Cite this

17A, a novel non-coding RNA, regulates GABA B alternative splicing and signaling in response to inflammatory stimuli and in Alzheimer disease. / Massone, Sara; Vassallo, Irene; Fiorino, Gloria; Castelnuovo, Manuele; Barbieri, Federica; Borghi, Roberta; Tabaton, Massimo; Robello, Mauro; Gatta, Elena; Russo, Claudio; Florio, Tullio; Dieci, Giorgio; Cancedda, Ranieri; Pagano, Aldo.

In: Neurobiology of Disease, Vol. 41, No. 2, 02.2011, p. 308-317.

Research output: Contribution to journalArticle

Massone, S, Vassallo, I, Fiorino, G, Castelnuovo, M, Barbieri, F, Borghi, R, Tabaton, M, Robello, M, Gatta, E, Russo, C, Florio, T, Dieci, G, Cancedda, R & Pagano, A 2011, '17A, a novel non-coding RNA, regulates GABA B alternative splicing and signaling in response to inflammatory stimuli and in Alzheimer disease', Neurobiology of Disease, vol. 41, no. 2, pp. 308-317. https://doi.org/10.1016/j.nbd.2010.09.019
Massone, Sara ; Vassallo, Irene ; Fiorino, Gloria ; Castelnuovo, Manuele ; Barbieri, Federica ; Borghi, Roberta ; Tabaton, Massimo ; Robello, Mauro ; Gatta, Elena ; Russo, Claudio ; Florio, Tullio ; Dieci, Giorgio ; Cancedda, Ranieri ; Pagano, Aldo. / 17A, a novel non-coding RNA, regulates GABA B alternative splicing and signaling in response to inflammatory stimuli and in Alzheimer disease. In: Neurobiology of Disease. 2011 ; Vol. 41, No. 2. pp. 308-317.
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