Mouse double minute 2 (MDM2) gene product is a protein that binds to and presumably inactivates the growth suppressive effects of wild-type p53 protein. The MDM2 gene has been found amplified in adult series of human gliomas, in absence of p53 mutation. Several studies have confirmed the expression of Platelet Derived Growth Factor (PDGF) and PDFG-Receptor-α (PDGFR-α) in adult gliomas. PDGF and its receptors have been implicated in normal development of the CNS as well as in neoplastic progression. PDGFR-α and Epidermal Growth Factor (EGFR) genes amplification have been found in different subsets of glioblastomas in adult series. To determine a role of MDM2 and PDGFR-α in pediatric gliomas we investigated the presence of MDM-2 and PDGFR-α genes amplification in a 20-cases series of childhood brain tumors biopsies that had been previously studied for the presence of EGFR amplification (and p53 mutations). We used differential PCR assay on DNA extracted from paraffin embedded or frozen tissues. Preliminary data show that the presence of MDM2 amplification is restricted to malignant gliomas that are negative for EGFR amplification, while PDGFR-α amplification was not detected in the tumors tested. Our results suggest that similar molecular mechanisms may operate in pediatric gliomas and in adult gliomas, even though their significance in relation to prognosis may be different.
|Number of pages||1|
|Journal||Italian Journal of Neurological Sciences|
|Publication status||Published - 1997|
ASJC Scopus subject areas
- Clinical Neurology