19p Deletion in recurring leiomyosarcoma lesions from the same patient

Paola Riva, Leda Dalprà, Valter Gualandri, Marinella Volontè, Monica Miozzo, Roberta Malgara, Annamaria Fuhrman Conti, Lidia Larizza

Research output: Contribution to journalArticle

Abstract

Ten leiomyosarcomas (LMS) affecting the same patient over a period of 3 years were cytogenetically studied to detect nonrandom chromosomal changes with a pathogenetic significance. All tumors, likely metastases of a previous LMS presentation, were classified as small, including eight that developed before chemotherapy; the diagnoses were based on standard immunohistochemistry methods for smooth muscle tumors. Scoring of 613 metaphases revealed monosomy of chromosome 22 in six LMS, monosomy of chromosome 19 in three, and deletion of chromosome 19p in all ten. Interphase fluorescence in situ hybridization (FISH) of the 22-alphoid-specific probe allowed loss of the target chromosome to be detected in four tumors at higher frequencies than those detected by cytogenetics. Double-color FISH of the 19p- and 19q-specific YAC performed on one tumor made it possible to distinguish the monosomic and 19p deleted cells, the relative frequencies of which were found to be 10% and 20%, respectively. The deletion breakpoint could be mapped at 19p13 between YAC 957d12 and YAC 947g4. The recurrence of the 19p deletion in a subset of tumor cells from all of the analyzed LMS suggests that this structural aberration is a significant change in the development of leiomyosarcomas. Copyright (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)102-108
Number of pages7
JournalCancer Genetics and Cytogenetics
Volume119
Issue number2
DOIs
Publication statusPublished - Jun 2000

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

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