[2-14C]caffeine metabolism in control and 3-methylcholanthrene induced rat liver microsomes by high pressure liquid chromatography

M. Bonati, R. Latini, E. Marzi

Research output: Contribution to journalArticle

Abstract

Theobromine, theophylline, paraxanthine and 1,3,7-trimethyluric acid were identified as caffeine metabolites after incubation of [2-14C]caffeine with rat liver microsomes and separation by high-pressure liquid chromatography (HPLC). A 9-fold induction of caffeine metabolism was observed in 3-methylcholanthrene (MC)-induced microsomes and this induction ranged from 4 to 11 times for individual metabolites. Induction of aryl hydrocarbon hydroxylase (AHH) was about 5-fold, comparable to that of theophylline formation. Primary caffeine metabolism is an enzymic process catalyzed by the microsomal mixed-function oxidases.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalToxicology Letters
Volume7
Issue number1
DOIs
Publication statusPublished - 1980

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High pressure liquid chromatography
Methylcholanthrene
Liver Microsomes
Caffeine
Metabolism
Liver
Rats
High Pressure Liquid Chromatography
Theophylline
Metabolites
Theobromine
Aryl Hydrocarbon Hydroxylases
Microsomes
Mixed Function Oxygenases

ASJC Scopus subject areas

  • Toxicology

Cite this

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abstract = "Theobromine, theophylline, paraxanthine and 1,3,7-trimethyluric acid were identified as caffeine metabolites after incubation of [2-14C]caffeine with rat liver microsomes and separation by high-pressure liquid chromatography (HPLC). A 9-fold induction of caffeine metabolism was observed in 3-methylcholanthrene (MC)-induced microsomes and this induction ranged from 4 to 11 times for individual metabolites. Induction of aryl hydrocarbon hydroxylase (AHH) was about 5-fold, comparable to that of theophylline formation. Primary caffeine metabolism is an enzymic process catalyzed by the microsomal mixed-function oxidases.",
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AU - Marzi, E.

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N2 - Theobromine, theophylline, paraxanthine and 1,3,7-trimethyluric acid were identified as caffeine metabolites after incubation of [2-14C]caffeine with rat liver microsomes and separation by high-pressure liquid chromatography (HPLC). A 9-fold induction of caffeine metabolism was observed in 3-methylcholanthrene (MC)-induced microsomes and this induction ranged from 4 to 11 times for individual metabolites. Induction of aryl hydrocarbon hydroxylase (AHH) was about 5-fold, comparable to that of theophylline formation. Primary caffeine metabolism is an enzymic process catalyzed by the microsomal mixed-function oxidases.

AB - Theobromine, theophylline, paraxanthine and 1,3,7-trimethyluric acid were identified as caffeine metabolites after incubation of [2-14C]caffeine with rat liver microsomes and separation by high-pressure liquid chromatography (HPLC). A 9-fold induction of caffeine metabolism was observed in 3-methylcholanthrene (MC)-induced microsomes and this induction ranged from 4 to 11 times for individual metabolites. Induction of aryl hydrocarbon hydroxylase (AHH) was about 5-fold, comparable to that of theophylline formation. Primary caffeine metabolism is an enzymic process catalyzed by the microsomal mixed-function oxidases.

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