Abstract
Theobromine, theophylline, paraxanthine and 1,3,7-trimethyluric acid were identified as caffeine metabolites after incubation of [2-14C]caffeine with rat liver microsomes and separation by high-pressure liquid chromatography (HPLC). A 9-fold induction of caffeine metabolism was observed in 3-methylcholanthrene (MC)-induced microsomes and this induction ranged from 4 to 11 times for individual metabolites. Induction of aryl hydrocarbon hydroxylase (AHH) was about 5-fold, comparable to that of theophylline formation. Primary caffeine metabolism is an enzymic process catalyzed by the microsomal mixed-function oxidases.
| Original language | English |
|---|---|
| Pages (from-to) | 1-7 |
| Number of pages | 7 |
| Journal | Toxicology Letters |
| Volume | 7 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1980 |
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ASJC Scopus subject areas
- Toxicology
Cite this
[2-14C]caffeine metabolism in control and 3-methylcholanthrene induced rat liver microsomes by high pressure liquid chromatography. / Bonati, M.; Latini, R.; Marzi, E.
In: Toxicology Letters, Vol. 7, No. 1, 1980, p. 1-7.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - [2-14C]caffeine metabolism in control and 3-methylcholanthrene induced rat liver microsomes by high pressure liquid chromatography
AU - Bonati, M.
AU - Latini, R.
AU - Marzi, E.
PY - 1980
Y1 - 1980
N2 - Theobromine, theophylline, paraxanthine and 1,3,7-trimethyluric acid were identified as caffeine metabolites after incubation of [2-14C]caffeine with rat liver microsomes and separation by high-pressure liquid chromatography (HPLC). A 9-fold induction of caffeine metabolism was observed in 3-methylcholanthrene (MC)-induced microsomes and this induction ranged from 4 to 11 times for individual metabolites. Induction of aryl hydrocarbon hydroxylase (AHH) was about 5-fold, comparable to that of theophylline formation. Primary caffeine metabolism is an enzymic process catalyzed by the microsomal mixed-function oxidases.
AB - Theobromine, theophylline, paraxanthine and 1,3,7-trimethyluric acid were identified as caffeine metabolites after incubation of [2-14C]caffeine with rat liver microsomes and separation by high-pressure liquid chromatography (HPLC). A 9-fold induction of caffeine metabolism was observed in 3-methylcholanthrene (MC)-induced microsomes and this induction ranged from 4 to 11 times for individual metabolites. Induction of aryl hydrocarbon hydroxylase (AHH) was about 5-fold, comparable to that of theophylline formation. Primary caffeine metabolism is an enzymic process catalyzed by the microsomal mixed-function oxidases.
UR - http://www.scopus.com/inward/record.url?scp=0019225215&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019225215&partnerID=8YFLogxK
U2 - 10.1016/0378-4274(80)90077-6
DO - 10.1016/0378-4274(80)90077-6
M3 - Article
C2 - 7292511
AN - SCOPUS:0019225215
VL - 7
SP - 1
EP - 7
JO - Toxicology Letters
JF - Toxicology Letters
SN - 0378-4274
IS - 1
ER -