2-D protein maps of rat gastrocnemius and soleus muscles: A tool for muscle plasticity assessment

Cecilia Gelfi, Agnese Viganò, Sara De Palma, Marilena Ripamonti, Shajna Begum, Paolo Cerretelli, Robin Wait

Research output: Contribution to journalArticlepeer-review

Abstract

Functional characterization of muscle fibers relies on ATPase activity and on differential measurements of metabolic proteins, including mitochondrial and glycolytic enzymes, glucose, lactate and lactic acid transporters, calcium cycling proteins and components of the contractile machinery. The recent introduction of microarray technology has enabled detailed gene expression studies under different physiological and pathological conditions, thus generating novel hypotheses on muscle function. However, microarray approaches are limited by the incomplete genome coverage of currently available chips, and by poor correlation between mRNA concentration and protein expression level. We have used 2-DE and MS to build a reference map of proteins from rat mixed gastrocnemius and soleus muscle, and to assess qualitative and quantitative differences in protein distribution between these two functionally dissimilar muscles. More than 800 spots on each gel were detected by silver staining, of which 167 were excised, digested in-gel with trypsin and analyzed by ESI-MS/MS. One hundred and twenty eight distinct gene products were identified, including metabolic, transport and contractile proteins. Forty one spots displayed differences in relative expression level between mixed gastrocnemius and soleus samples. These data not only enable differentiation of functionally distinct slow-twitch and fast-twitch fiber types, but also provide tools for investigating muscle plasticity in response to physiological and environmental conditions such as aging or hypoxia.

Original languageEnglish
Pages (from-to)321-340
Number of pages20
JournalProteomics
Volume6
Issue number1
DOIs
Publication statusPublished - Jan 2006

Keywords

  • 2-DE
  • Aging
  • MS
  • Skeletal muscle plasticity

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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