2-DE and LC-MS/MS for a comparative proteomic analysis of balf from subjects with different subsets of inflammatory myopathies

Ileana Passadore, Paolo Iadarola, Cristina Di Poto, Serena Giuliano, Carlomaurizio Montecucco, Lorenzo Cavagna, Claudia Bonino, Federica Meloni, Anna Maria Fietta, Antonella Lisa, Roberta Salvini, Anna Maria Bardoni

Research output: Contribution to journalArticlepeer-review

Abstract

The protein profiles of bronchoalveolar lavage fluid (BALf) of patients belonging to three selected subsets of Polymyositis/Dermatomyositis (PM/DM) have been compared by using a combination of 2-DE and MALDI-TOF/MS or LC-MS/MS. Our study examined the hypothesis that there were distinct differences in protein expression profiles that were related to the phenotype. From among the 323 ± 51 protein spots that may represent the most highly expressed proteins in BALf of these patients, 24 unique spots were isolated and proteins identified. In particular, 9 spots were present in BALf of PM/DM patients only; 12 spots were exclusive of Overlap patients and 3 spots of AS patients. From among the proteins identified, a few were classified as cytoskeletal proteins, others were involved in oxidative stress and a number of proteins were associated with general metabolic activity or immunological response and inflammation. This is the first study in which evidence is provided that a number of different proteins are expressed in different subsets of PM/DM and supports our contention that the proteomic approach would be beneficial in discovering molecules which could represent possible prognostic factors of these rare pathologies.

Original languageEnglish
Pages (from-to)2331-2340
Number of pages10
JournalJournal of Proteome Research
Volume8
Issue number5
DOIs
Publication statusPublished - May 1 2009

Keywords

  • 2-DE
  • AS
  • LC-MS/MS
  • Overlap syndrome
  • PM/DM

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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