Abstract
The protein profiles of bronchoalveolar lavage fluid (BALf) of patients belonging to three selected subsets of Polymyositis/Dermatomyositis (PM/DM) have been compared by using a combination of 2-DE and MALDI-TOF/MS or LC-MS/MS. Our study examined the hypothesis that there were distinct differences in protein expression profiles that were related to the phenotype. From among the 323 ± 51 protein spots that may represent the most highly expressed proteins in BALf of these patients, 24 unique spots were isolated and proteins identified. In particular, 9 spots were present in BALf of PM/DM patients only; 12 spots were exclusive of Overlap patients and 3 spots of AS patients. From among the proteins identified, a few were classified as cytoskeletal proteins, others were involved in oxidative stress and a number of proteins were associated with general metabolic activity or immunological response and inflammation. This is the first study in which evidence is provided that a number of different proteins are expressed in different subsets of PM/DM and supports our contention that the proteomic approach would be beneficial in discovering molecules which could represent possible prognostic factors of these rare pathologies.
Original language | English |
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Pages (from-to) | 2331-2340 |
Number of pages | 10 |
Journal | Journal of Proteome Research |
Volume | 8 |
Issue number | 5 |
DOIs | |
Publication status | Published - May 1 2009 |
Keywords
- 2-DE
- AS
- LC-MS/MS
- Overlap syndrome
- PM/DM
ASJC Scopus subject areas
- Biochemistry
- Chemistry(all)