2-deoxy-D-ribose-induced apoptosis in HL-60 cells is associated with the cell cycle progression by spermidine

Maria Giuseppina Monti, Stefania Ghiaroni, Daniela Barbieri, Claudio Franceschi, Gaetano Marverti, Maria Stella Moruzzi

Research output: Contribution to journalArticle

Abstract

The presence of polyamines is required for the apoptotic program triggered by 2-deoxy-D-ribose (dRib) in HL-60 cells, but their oxidative metabolites does not appear to be involved in the oxidative stress caused by the sugar. The present study points to a relationship between spermidine-induced G1 to S phase transition and the onset of dRib-induced apoptosis. Conversely, the G1 block induced by α-difluoromethylornithine (DFMO) is associated with a protective effect against dRib-induced cell suicide. Replenishment of the intracellular spermidine pool by exogenous putrescine and spermidine induces cell cycle progression and restores apoptotic levels. The present data indicate that the induction of cell cycle progression by spermidine is a condition facilitating the activation of the apoptotic process by dRib.

Original languageEnglish
Pages (from-to)460-465
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume257
Issue number2
DOIs
Publication statusPublished - Apr 13 1999

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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