200+ Protein Concentrations in Healthy Human Blood Plasma: Targeted Quantitative SRM SIS Screening of Chromosomes 18, 13, Y and the Mitochondrial Chromosome Encoded Proteome

Arthur T Kopylov, Elena A Ponomarenko, Ekaterina V Ilgisonis, Mikhail A Pyatnitskiy, Andrey V Lisitsa, Ekaterina V Poverennaya, Olga I Kiseleva, Tatiana E Farafonova, Olga V Tikhonova, Maria G Zavialova, Svetlana Novikova, Sergei A Moshkovskii, Sergey P Radko, Boris V Morukov, Anatoly I Grigoriev, Young-Ki Paik, Ghasem Hosseini Salekdeh, Andrea Urbani, Victor G Zgoda, Alexander I Archakov

Research output: Contribution to journalArticle

Abstract

This work continues the series of the quantitative measurements of the proteins encoded by different chromosomes in the blood plasma of a healthy person. Selected Reaction Monitoring with Stable Isotope-labeled peptide Standards (SRM SIS) and a gene-centric approach, which is the basis for the implementation of the international Chromosome-centric Human Proteome Project (C-HPP), were applied for the quantitative measurement of proteins in human blood plasma. Analyses were carried out in the frame of C-HPP for each protein-coding gene of the four human chromosomes: 18, 13, Y and mitochondrial. Concentrations of proteins encoded by 667 genes were measured in 54 blood plasma samples of the volunteers, which health conditions were consistent with requirements to the astronauts. The gene list included 276, 329, 47 and 15 genes of chromosomes 18, 13, and Y and the mitochondrial chromosome, respectively. Only 205 proteins (30.7%) were detected in the samples. Of them, 84, 106, 10 and 5 belonged to chromosomes 18, 13, and Y and the mitochondrial chromosome, respectively. Each detected protein was found in at least one of the samples analyzed. The SRM SIS raw data are available in the ProteomeXchange repository (PXD004374, PASS01192).

Original languageEnglish
JournalJournal of Proteome Research
DOIs
Publication statusE-pub ahead of print - Nov 27 2018

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    Kopylov, A. T., Ponomarenko, E. A., Ilgisonis, E. V., Pyatnitskiy, M. A., Lisitsa, A. V., Poverennaya, E. V., Kiseleva, O. I., Farafonova, T. E., Tikhonova, O. V., Zavialova, M. G., Novikova, S., Moshkovskii, S. A., Radko, S. P., Morukov, B. V., Grigoriev, A. I., Paik, Y-K., Salekdeh, G. H., Urbani, A., Zgoda, V. G., & Archakov, A. I. (2018). 200+ Protein Concentrations in Healthy Human Blood Plasma: Targeted Quantitative SRM SIS Screening of Chromosomes 18, 13, Y and the Mitochondrial Chromosome Encoded Proteome. Journal of Proteome Research. https://doi.org/10.1021/acs.jproteome.8b00391