TY - JOUR
T1 - 2,3,7,8-Tetrachlorodibenzo-p-dioxin regulates Bovine Herpesvirus type 1 induced apoptosis by modulating Bcl-2 family members
AU - Fiorito, Filomena
AU - Marfè, Gabriella
AU - De Blasio, Emma
AU - Granato, Giovanna Elvira
AU - Tafani, Marco
AU - De Martino, Luisa
AU - Montagnaro, Serena
AU - Florio, Salvatore
AU - Pagnini, Ugo
PY - 2008/10
Y1 - 2008/10
N2 - Exposure to environmental contaminants, like 2,3,7,8-tetrachlorodibenzo-p- dioxin (TCDD), leads to an increased susceptibility to infectious agents. Infection of bovine cells (MDBK) with Bovine Herpesvirus 1 (BHV-1) anticipates virus-induced apoptosis, suggesting an involvement of TCDD in virus infection. Herein we analyzed the effects of TCDD on apoptotic pathway in MDBK cells infected with BHV-1. After 12 h of infection, TCDD induced a significant increase in apoptotic cells. TCDD caused a dose-dependent up-regulation and anticipated activation of caspases 3, 8 and 9, with respect to unexposed groups. TCDD anticipated cleavage of PARP, compared to controls. Furthermore TCDD increased Bax and Bid levels, and decreased Bcl-2 and Bcl-XL levels. Such events took place earlier in exposed than unexposed cells. These results showed that TCDD influences BHV-1 induced apoptosis through members of Bcl-2 family and up-regulating activation of caspases.
AB - Exposure to environmental contaminants, like 2,3,7,8-tetrachlorodibenzo-p- dioxin (TCDD), leads to an increased susceptibility to infectious agents. Infection of bovine cells (MDBK) with Bovine Herpesvirus 1 (BHV-1) anticipates virus-induced apoptosis, suggesting an involvement of TCDD in virus infection. Herein we analyzed the effects of TCDD on apoptotic pathway in MDBK cells infected with BHV-1. After 12 h of infection, TCDD induced a significant increase in apoptotic cells. TCDD caused a dose-dependent up-regulation and anticipated activation of caspases 3, 8 and 9, with respect to unexposed groups. TCDD anticipated cleavage of PARP, compared to controls. Furthermore TCDD increased Bax and Bid levels, and decreased Bcl-2 and Bcl-XL levels. Such events took place earlier in exposed than unexposed cells. These results showed that TCDD influences BHV-1 induced apoptosis through members of Bcl-2 family and up-regulating activation of caspases.
KW - Bcl-2
KW - BHV-1
KW - Caspases
KW - PARP
KW - TCDD
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UR - http://www.scopus.com/inward/citedby.url?scp=51749104414&partnerID=8YFLogxK
U2 - 10.1007/s10495-008-0249-y
DO - 10.1007/s10495-008-0249-y
M3 - Article
C2 - 18696228
AN - SCOPUS:51749104414
VL - 13
SP - 1243
EP - 1252
JO - Apoptosis : an international journal on programmed cell death
JF - Apoptosis : an international journal on programmed cell death
SN - 1360-8185
IS - 10
ER -