23Na-NMR study of cation cotransport in human red blood cells

M. Cacciafesta, V. Marigliano, C. Ferri, G. Piccirillo, A. Scuteri, L. Guidoni, A. M. Luciani, A. Rosi, V. Viti

Research output: Contribution to journalArticle

Abstract

Na+-K+ cotransport in human erythrocytes from healthy subjects has been studied by means of 23Na-nuclear magnetic resonance (NMR) in the presence of the anionic paramagnetic shift reagent dysprosium (III) tripolyphosphate [Dy(PPP)2/7-]. The intra- and extracellular 23Na-NMR signals were well separated, giving values of 6 ± 1 mM for internal sodium concentration. Determination of the furosemide-sensitive Na+ efflux, in the presence of ouabain, was obtained by correlating the chemical shift variation of the external signal with changes in external Na+ concentration. For this purpose, calibration curves were generated. The values of the maximum efflux velocity (V(max); 0.29-1 mmol · h-1 · 1 cells-1) measured in 10 healthy adult male subjects were found to be within the range of values obtained by other authors. The NMR method proposed for the study of Na+-K+ cotransport is relatively simple and allows quick evaluation of cotransport V(max) values for a number of samples, being a good candidate for the study of this transport mechanism in the presence of different pathologies.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume262
Issue number5 31-5
Publication statusPublished - 1992

Keywords

  • normal human erythrocytes
  • ouabain-insensitive sodium effluxes
  • sodium-potassium transport mechanisms

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology
  • Medicine(all)

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  • Cite this

    Cacciafesta, M., Marigliano, V., Ferri, C., Piccirillo, G., Scuteri, A., Guidoni, L., Luciani, A. M., Rosi, A., & Viti, V. (1992). 23Na-NMR study of cation cotransport in human red blood cells. American Journal of Physiology - Cell Physiology, 262(5 31-5).