25-hydroxycholesterol reduces inflammation, viral load and cell death in ZIKV-infected U-87 MG glial cell line

Paola Maura Tricarico, Ilaria Caracciolo, Rossella Gratton, Pierlanfranco D’Agaro, Sergio Crovella

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Zika virus (ZIKV) infection is a global health issue due to its worldwide diffusion and to the related effects on neural progenitor cells with severe consequences on developing brain as well as on the central nervous system of adults. Previous studies showed that ZIKV infection induces an increment of IL1B expression in the central nervous system and also in the blood. IL-1β is a pro-inflammatory cytokine essential for cellular defence, tissue repair and neuroinflammation, a mechanism seen to be associated with several neuroinflammatory diseases. 25-hydroxycholesterol (25-HC) is a natural oxysterol, derived from hydroxylation of cholesterol, possessing important antiviral activity possibly correlated to its ability to alter host membrane structures. Furthermore, 25-HC is involved in the modulation of IL1B gene expression, being able to suppress IL-1β driven inflammation probably by blocking the activation of the SREB proteins. In our study, we analysed the antiviral action of 25-HC in ZIKV-infected U-87 MG cells, also evaluating its impact on inflammation and cell death. We demonstrated that 25-HC is able to reduce inflammation and cell death caused by ZIKV infection and also to diminish intracellular ZIKV load in U-87 MG glial cell line. Considering its antiviral activity and its ability to penetrate blood–brain barrier, 25-HC could be proposed, based on our results and literature findings, as a potential anti-ZIKV agent.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalInflammopharmacology
DOIs
Publication statusAccepted/In press - Jul 17 2018

Fingerprint

Viral Load
Neuroglia
Cell Death
Inflammation
Cell Line
Antiviral Agents
Interleukin-1
Central Nervous System
Hydroxylation
Stem Cells
Cholesterol
25-hydroxycholesterol
Zika Virus
Cytokines
Gene Expression
Membranes
Brain
Zika Virus Infection
Proteins

Keywords

  • 25-hydroxycholesterol
  • Inflammation
  • U-87 MG glial cell line
  • ZIKV

ASJC Scopus subject areas

  • Immunology
  • Pharmacology
  • Pharmacology (medical)

Cite this

25-hydroxycholesterol reduces inflammation, viral load and cell death in ZIKV-infected U-87 MG glial cell line. / Tricarico, Paola Maura; Caracciolo, Ilaria; Gratton, Rossella; D’Agaro, Pierlanfranco; Crovella, Sergio.

In: Inflammopharmacology, 17.07.2018, p. 1-5.

Research output: Contribution to journalArticle

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