2D-A nd 3D-cultures of human trabecular meshwork cells: A preliminary assessment of an in vitro model for glaucoma study

Stefania Vernazza, Sara Tirendi, Sonia Scarfi, Mario Passalacqua, Francesco Oddone, Carlo Enrico Traverso, Ilaria Rizzato, Anna Maria Bassi, Sergio Claudio Sacca

Research output: Contribution to journalArticle

Abstract

A physiologically relevant in vitro human-based model could be the 'gold standard' to clarify the pathological steps involved in glaucoma onset. In this regard, human 3D cultures may represent an excellent starting point to achieve this goal. Indeed, the 3D matrix allows to recreate the in vivo-like tissue architecture, maintaining its functionality and cellular behaviour, compared to the 2D model. Thus, we propose a comparison between the 2D and 3D in vitro models of human trabecular meshwork cells in terms of cellular responses after chronic stress exposure. Our results showed that 3D-cells are more sensitive to intracellular reactive oxidative specie production induced by hydrogen peroxide treatment, compared to 2D cultures. Additionally, in 3D cultures a more accurate regulation of the apoptosis trigger and cell adaptation mechanisms was detected than in 2D models. In line with these findings, the 3D-HTMC model shows the ability to better mimic the in vivo cell behaviour in adaptive responses to chronic oxidative stress than 2D.

Original languageEnglish
Article numbere0221942
JournalPLoS One
Volume14
Issue number9
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Trabecular Meshwork
glaucoma
Glaucoma
cells
Aptitude
Psychological Adaptation
Oxidative stress
Hydrogen Peroxide
Oxidative Stress
gold
hydrogen peroxide
Apoptosis
oxidative stress
apoptosis
In Vitro Techniques
Tissue

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

2D-A nd 3D-cultures of human trabecular meshwork cells : A preliminary assessment of an in vitro model for glaucoma study. / Vernazza, Stefania; Tirendi, Sara; Scarfi, Sonia; Passalacqua, Mario; Oddone, Francesco; Traverso, Carlo Enrico; Rizzato, Ilaria; Bassi, Anna Maria; Sacca, Sergio Claudio.

In: PLoS One, Vol. 14, No. 9, e0221942, 01.01.2019.

Research output: Contribution to journalArticle

Vernazza, Stefania ; Tirendi, Sara ; Scarfi, Sonia ; Passalacqua, Mario ; Oddone, Francesco ; Traverso, Carlo Enrico ; Rizzato, Ilaria ; Bassi, Anna Maria ; Sacca, Sergio Claudio. / 2D-A nd 3D-cultures of human trabecular meshwork cells : A preliminary assessment of an in vitro model for glaucoma study. In: PLoS One. 2019 ; Vol. 14, No. 9.
@article{10c33e0a38154964962ccaed2a2df6cd,
title = "2D-A nd 3D-cultures of human trabecular meshwork cells: A preliminary assessment of an in vitro model for glaucoma study",
abstract = "A physiologically relevant in vitro human-based model could be the 'gold standard' to clarify the pathological steps involved in glaucoma onset. In this regard, human 3D cultures may represent an excellent starting point to achieve this goal. Indeed, the 3D matrix allows to recreate the in vivo-like tissue architecture, maintaining its functionality and cellular behaviour, compared to the 2D model. Thus, we propose a comparison between the 2D and 3D in vitro models of human trabecular meshwork cells in terms of cellular responses after chronic stress exposure. Our results showed that 3D-cells are more sensitive to intracellular reactive oxidative specie production induced by hydrogen peroxide treatment, compared to 2D cultures. Additionally, in 3D cultures a more accurate regulation of the apoptosis trigger and cell adaptation mechanisms was detected than in 2D models. In line with these findings, the 3D-HTMC model shows the ability to better mimic the in vivo cell behaviour in adaptive responses to chronic oxidative stress than 2D.",
author = "Stefania Vernazza and Sara Tirendi and Sonia Scarfi and Mario Passalacqua and Francesco Oddone and Traverso, {Carlo Enrico} and Ilaria Rizzato and Bassi, {Anna Maria} and Sacca, {Sergio Claudio}",
year = "2019",
month = "1",
day = "1",
doi = "10.1371/journal.pone.0221942",
language = "English",
volume = "14",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "9",

}

TY - JOUR

T1 - 2D-A nd 3D-cultures of human trabecular meshwork cells

T2 - A preliminary assessment of an in vitro model for glaucoma study

AU - Vernazza, Stefania

AU - Tirendi, Sara

AU - Scarfi, Sonia

AU - Passalacqua, Mario

AU - Oddone, Francesco

AU - Traverso, Carlo Enrico

AU - Rizzato, Ilaria

AU - Bassi, Anna Maria

AU - Sacca, Sergio Claudio

PY - 2019/1/1

Y1 - 2019/1/1

N2 - A physiologically relevant in vitro human-based model could be the 'gold standard' to clarify the pathological steps involved in glaucoma onset. In this regard, human 3D cultures may represent an excellent starting point to achieve this goal. Indeed, the 3D matrix allows to recreate the in vivo-like tissue architecture, maintaining its functionality and cellular behaviour, compared to the 2D model. Thus, we propose a comparison between the 2D and 3D in vitro models of human trabecular meshwork cells in terms of cellular responses after chronic stress exposure. Our results showed that 3D-cells are more sensitive to intracellular reactive oxidative specie production induced by hydrogen peroxide treatment, compared to 2D cultures. Additionally, in 3D cultures a more accurate regulation of the apoptosis trigger and cell adaptation mechanisms was detected than in 2D models. In line with these findings, the 3D-HTMC model shows the ability to better mimic the in vivo cell behaviour in adaptive responses to chronic oxidative stress than 2D.

AB - A physiologically relevant in vitro human-based model could be the 'gold standard' to clarify the pathological steps involved in glaucoma onset. In this regard, human 3D cultures may represent an excellent starting point to achieve this goal. Indeed, the 3D matrix allows to recreate the in vivo-like tissue architecture, maintaining its functionality and cellular behaviour, compared to the 2D model. Thus, we propose a comparison between the 2D and 3D in vitro models of human trabecular meshwork cells in terms of cellular responses after chronic stress exposure. Our results showed that 3D-cells are more sensitive to intracellular reactive oxidative specie production induced by hydrogen peroxide treatment, compared to 2D cultures. Additionally, in 3D cultures a more accurate regulation of the apoptosis trigger and cell adaptation mechanisms was detected than in 2D models. In line with these findings, the 3D-HTMC model shows the ability to better mimic the in vivo cell behaviour in adaptive responses to chronic oxidative stress than 2D.

UR - http://www.scopus.com/inward/record.url?scp=85071988936&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85071988936&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0221942

DO - 10.1371/journal.pone.0221942

M3 - Article

C2 - 31490976

AN - SCOPUS:85071988936

VL - 14

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 9

M1 - e0221942

ER -