2D immunomic approach for the study of IgG autoantibodies in the experimental model of multiple sclerosis

Alessia Farinazzo, Beatrice Gini, Alberto Milli, Francesca Ruffini, Silvia Marconi, Ermanna Turano, Elena Anghileri, Francesca Barbieri, Daniela Cecconi, Roberto Furlan, Bruno Bonetti

Research output: Contribution to journalArticlepeer-review

Abstract

2D-immunomics may be useful in the identification of autoantigens in neurological autoimmune diseases, but its application may be limited by denaturation of target proteins. Here we compared the capacity of a single or multiple antigens to elicit autoantibodies targeting multiple neural autoantigens by ELISA and 2D-immunomics. We induced experimental autoimmune encephalomyelitis (EAE) with MBP peptide89-104, total MBP or spinal cord homogenate. Both techniques showed anti-MBP IgG only after immunization with total MBP. In addition, 2D-immunomics revealed the presence in EAE mice of autoantibodies targeting other neural proteins, some displaying partial sequence homology with MBP. The present finding by 2D-immunomics of multiple neural proteins targeted by autoantibodies generated by a single antigen may help to explain the complex autoimmune response observed in multiple sclerosis.

Original languageEnglish
Pages (from-to)63-67
Number of pages5
JournalJournal of Neuroimmunology
Volume232
Issue number1-2
DOIs
Publication statusPublished - Mar 2011

Keywords

  • 2D-PAGE
  • Autoantibodies
  • ELISA
  • Experimental autoimmune encephalomyelitis
  • Immunomics
  • Molecular mimicry

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

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