2D immunomic approach for the study of IgG autoantibodies in the experimental model of multiple sclerosis

Alessia Farinazzo; Beatrice Gini; Alberto Milli; Francesca Ruffini; Silvia Marconi; Ermanna Turano; Elena Anghileri; Francesca Barbieri; Daniela Cecconi; Roberto Furlan; Bruno Bonetti

doi:10.1016/j.jneuroim.2010.10.004

2D immunomic approach for the study of IgG autoantibodies in the experimental model of multiple sclerosis

Alessia Farinazzo, Beatrice Gini, Alberto Milli, Francesca Ruffini, Silvia Marconi, Ermanna Turano, Elena Anghileri, Francesca Barbieri, Daniela Cecconi, Roberto Furlan, Bruno Bonetti

Research output: Contribution to journal › Article › peer-review

Abstract

2D-immunomics may be useful in the identification of autoantigens in neurological autoimmune diseases, but its application may be limited by denaturation of target proteins. Here we compared the capacity of a single or multiple antigens to elicit autoantibodies targeting multiple neural autoantigens by ELISA and 2D-immunomics. We induced experimental autoimmune encephalomyelitis (EAE) with MBP peptide_89-104, total MBP or spinal cord homogenate. Both techniques showed anti-MBP IgG only after immunization with total MBP. In addition, 2D-immunomics revealed the presence in EAE mice of autoantibodies targeting other neural proteins, some displaying partial sequence homology with MBP. The present finding by 2D-immunomics of multiple neural proteins targeted by autoantibodies generated by a single antigen may help to explain the complex autoimmune response observed in multiple sclerosis.

Original language	English
Pages (from-to)	63-67
Number of pages	5
Journal	Journal of Neuroimmunology
Volume	232
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2010.10.004
Publication status	Published - Mar 2011

Keywords

2D-PAGE
Autoantibodies
ELISA
Experimental autoimmune encephalomyelitis
Immunomics
Molecular mimicry

ASJC Scopus subject areas

Immunology
Clinical Neurology
Immunology and Allergy
Neurology

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2D immunomic approach for the study of IgG autoantibodies in the experimental model of multiple sclerosis. / Farinazzo, Alessia; Gini, Beatrice; Milli, Alberto; Ruffini, Francesca; Marconi, Silvia; Turano, Ermanna; Anghileri, Elena; Barbieri, Francesca; Cecconi, Daniela; Furlan, Roberto; Bonetti, Bruno.

In: Journal of Neuroimmunology, Vol. 232, No. 1-2, 03.2011, p. 63-67.

Research output: Contribution to journal › Article › peer-review

Farinazzo, Alessia ; Gini, Beatrice ; Milli, Alberto ; Ruffini, Francesca ; Marconi, Silvia ; Turano, Ermanna ; Anghileri, Elena ; Barbieri, Francesca ; Cecconi, Daniela ; Furlan, Roberto ; Bonetti, Bruno. / 2D immunomic approach for the study of IgG autoantibodies in the experimental model of multiple sclerosis. In: Journal of Neuroimmunology. 2011 ; Vol. 232, No. 1-2. pp. 63-67.

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abstract = "2D-immunomics may be useful in the identification of autoantigens in neurological autoimmune diseases, but its application may be limited by denaturation of target proteins. Here we compared the capacity of a single or multiple antigens to elicit autoantibodies targeting multiple neural autoantigens by ELISA and 2D-immunomics. We induced experimental autoimmune encephalomyelitis (EAE) with MBP peptide89-104, total MBP or spinal cord homogenate. Both techniques showed anti-MBP IgG only after immunization with total MBP. In addition, 2D-immunomics revealed the presence in EAE mice of autoantibodies targeting other neural proteins, some displaying partial sequence homology with MBP. The present finding by 2D-immunomics of multiple neural proteins targeted by autoantibodies generated by a single antigen may help to explain the complex autoimmune response observed in multiple sclerosis.",

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AU - Turano, Ermanna

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