2de maps in the discovery of human autoimmune kidney diseases: The case of membranous glomerulonephritis

Maurizio Bruschi, Laura Santucci, Gian Marco Ghiggeri, Giovanni Candiano

Research output: Contribution to journalArticle

Abstract

The identification of antigens in the autoimmune diseases is a primary point to elucidate the pathogenesis of disease. Here, we propose an “in vivo” proteomics approach to identify the antigens of auto-antibodies in membranous glomerulonephritis. In this approach, podocyte proteins resolved by two-dimensional electrophoresis were semidry blotted to nitrocellulose membrane. Then the antibodies eluted from microdissected glomeruli and serum samples were used as a probe for the detection of podocyte antigens and characterized by means of mass spectrometry. These combined methods allowed us to identify six new antigens in membranous glomerulonephritis.

Original languageEnglish
Pages (from-to)127-138
Number of pages12
JournalMethods in molecular biology (Clifton, N.J.)
Volume1243
DOIs
Publication statusPublished - 2014

Keywords

  • Autoimmunity
  • Glomerular autoantibodies
  • Glomerulonephritis
  • Immunoblotting
  • Laser capture microdissection
  • Membranous nephropathy
  • Semidry western blot
  • Two-dimensional electrophoresis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

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