3-aminobenzamide protects cells from UV-B-induced apoptosis by acting on cytoskeleton and substrate adhesion

Walter Malorni, Roberte Rivabene, Elisabetta Straface, Gabriella Rainaldi, Daniela Monti, Stefano Salvioli, Andrea Cossarizza, Claudio Franceschi

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3-aminobenzamide (3-ABA) is an inhibitor of poly-(ADP-ribose)-polymerase, an enzyme involved in numerous subcellular processes, including cell death. Recently, a target effect of the drug on some cytoskeletal elements has also been described. In this study we evaluated the ability of 3-ABA to interfere with UV-B ray-induced apoptosis in cells selected for their cytoskeletal features and their different capability to adhere to the substrate. Human melanoma (M14) and epithelial (A431) cell lines and murine primary fibroblastic cultures (MFC) were studied. Our results indicate that cytoskeleton is indeed an important cellular target of 3-ABA, which can prevent apoptotic cell death by UV-B through a specific effect on the adhesion properties of the cells. Indeed, an inverse correlation was observed between sensitivity to UV-B-induced apoptosis (M14>A431>MFC) and substrate adhesion (MFC>A431>M14). The potential relevance of these observations to understand the possible relationships among apoptosis, cytoskeletal functions and substrate adhesion is discussed.

Original languageEnglish
Pages (from-to)715-724
Number of pages10
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - 1995

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

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