3-Aroyl-1,4-diarylpyrroles Inhibit Chronic Myeloid Leukemia Cell Growth through an Interaction with Tubulin

Giuseppe La Regina, Ruoli Bai, Antonio Coluccia, Valeria Famiglini, Sara Passacantilli, Valentina Naccarato, Giorgio Ortar, Carmela Mazzoccoli, Vitalba Ruggieri, Francesca Agriesti, Claudia Piccoli, Tiziana Tataranni, Marianna Nalli, Andrea Brancale, Stefania Vultaggio, Ciro Mercurio, Mario Varasi, Concetta Saponaro, Sara Sergio, Michele MaffiaAddolorata Maria Luce Coluccia, Ernest Hamel, Romano Silvestri

Research output: Contribution to journalArticle

Abstract

We designed 3-aroyl-1,4-diarylpyrrole (ARDAP) derivatives as potential anticancer agents having different substituents at the 1- or 4-phenyl ring. ARDAP compounds exhibited potent inhibition of tubulin polymerization, binding of colchicine to tubulin, and cancer cell growth. ARDAP derivative 10 inhibited the proliferation of BCR/ABL-expressing KU812 and LAMA84 cells from chronic myeloid leukemia (CML) patients in blast crisis and of hematopoietic cells ectopically expressing the imatinib mesylate (IM)-sensitive KBM5-WT or its IM-resistant KBM5-T315I mutation. Compound 10 minimally affected the proliferation of normal blood cells, indicating that it may be a promising agent to overcome broad tyrosine kinase inhibitor resistance in relapsed/refractory CML patients. Compound 10 significantly decreased CML proliferation by inducing G2/M phase arrest and apoptosis via a mitochondria-dependent pathway. ARDAP 10 augmented the cytotoxic effects of IM in human CML cells. Compound 10 represents a robust lead compound to develop tubulin inhibitors with potential as novel treatments for CML.

Original languageEnglish
Pages (from-to)521-526
Number of pages6
JournalACS Medicinal Chemistry Letters
Volume8
Issue number5
DOIs
Publication statusPublished - May 11 2017

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Keywords

  • 3-aroyl-1,4-diarylpyrrole
  • Cancer
  • chronic myeloid leukemia
  • synthesis
  • tubulin

ASJC Scopus subject areas

  • Biochemistry
  • Drug Discovery
  • Organic Chemistry

Cite this

La Regina, G., Bai, R., Coluccia, A., Famiglini, V., Passacantilli, S., Naccarato, V., Ortar, G., Mazzoccoli, C., Ruggieri, V., Agriesti, F., Piccoli, C., Tataranni, T., Nalli, M., Brancale, A., Vultaggio, S., Mercurio, C., Varasi, M., Saponaro, C., Sergio, S., ... Silvestri, R. (2017). 3-Aroyl-1,4-diarylpyrroles Inhibit Chronic Myeloid Leukemia Cell Growth through an Interaction with Tubulin. ACS Medicinal Chemistry Letters, 8(5), 521-526. https://doi.org/10.1021/acsmedchemlett.7b00022