3-Methyladenine-DNA-glycosylase and O6-alkyl guanine-DNA-alkyltransferase activities and sensitivity to alkylating agents in human cancer cell lines

G. Damia, L. Imperatori, L. Citti, L. Mariani, M. D'Incalci

Research output: Contribution to journalArticle

Abstract

The activities and the expression of 3-methyladenine glycosylase (3-meAde gly) and O6-alkylguanine-DNA-alkyltransferase (O6ATase) were investigated in ten human cancer cell lines. Both 3-meAde gly and O6ATase activities were variable among different cell lines. mRNA levels of the O6ATase gene, appeared to be related to the content of O6ATase in different cell lines, whereas no apparent correlation was found between mRNA of 3-meAde gly and the enzymatic activity. No correlation was found between the activity of the two enzymes and the sensitivity to alkylating agents of different structures such as CC-1065, tallimustine, dimethylsulphate (DMSO), N-methyl-N1-nitro-N-nitrosoguanidine (MNNG), cis-diamminedichloroplatinum (cDDP) and melphalan (L-PAM). The most striking finding of this study is that a correlation exists between the activity of O6ATase and 3-meAde gly in the various cell lines investigated (P <0.01), suggesting a common mechanism of regulation of two DNA repair enzymes.

Original languageEnglish
Pages (from-to)861-865
Number of pages5
JournalBritish Journal of Cancer
Volume73
Issue number7
Publication statusPublished - 1996

Keywords

  • 3-methyladenine glycosylase
  • Alkylating agents
  • O ATase

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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