3-methylcholanthrene induces differential inhibition of humoral and cell mediated immune responses in mice of different ages

Marina Sironi, Enrico Pesenti, Sergio Bernasconi, Annunciata Vecchi

Research output: Contribution to journalArticlepeer-review

Abstract

C57B1/6 mice aged 2-3 and 13-14 months were treated i.p. with 3-methylcholanthrene. A single dose of 25 mg/kg reduced primary antibody production to the T-dependent antigen sheep red blood cells by 20% in mice aged 2-3 months and by 90% in 13-14-month-old animals. The same treatment did not reduce antibody production to the T-independent antigen pneumococcal polysaccharide type IlI in young mice, but reduced this response by 50% in 13-14-month-old animals. Blastogenesis to concanavalin A, phytohemagglutinin and alloantigens, that is mediated by T lymphocytes, was consistently reduced in young animals but only marginally affected, when at all, in 13-14-month-old mice. Blastogenesis to lipolpolysaccharide, mediated by B lymphocytes, was reduced in mice of both ages, though in older mice it was affected later than in younger animals. Addition of 3-methylcholanthrene in vitro increased T lymphocyte responses equally in mice of both ages and did not modify B lymphocyte proliferation. Results presented here show that older mice are not necessarily more susceptible to all types of immunosuppression induced by a xenobiotic like 3-methylcholanthrene and that the sensitivity of the different facets of the immune response can change with aging.

Original languageEnglish
Pages (from-to)263-273
Number of pages11
JournalToxicology
Volume60
Issue number3
DOIs
Publication statusPublished - Mar 16 1990

Keywords

  • 3-Methylcholanthrene
  • Antibody production
  • Carcinogen
  • Cell-mediated responses

ASJC Scopus subject areas

  • Toxicology

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