32P-Postlabeling detection of DNA adducts in mice treated with the herbicide Roundup

Marco Peluso, Armelle Munnia, Claudia Bolognesi, Silvio Parodi

Research output: Contribution to journalArticle

Abstract

Roundup is a postemergence herbicide acting on the synthesis of amino acids and other important endogenous chemicals in plants. Roundup is commonly used in agriculture, forestry, and nurseries for the control or destruction of most herbaceous plants. The present study shows that Roundup is able to induce a dose-dependent formation of DNA adducts in the kidneys and liver of mice. The levels of Roundup-related DNA adducts observed in mouse kidneys and liver at the highest dose of herbicide tested (600 mg/kg) were 3.0 ± 0.1 (SE) and 1.7 ± 0.1 (SE) adducts/108 nucleotides, respectively. The Roundup DNA adducts were not related to the active ingredient, the isopropylammonium salt of glyphosate, but to another, unknown component of the herbicide mixture. Additional experiments are needed to identify the chemical specie(s) of Roundup mixture involved in DNA adducts formation. Findings of this study may help to protect agricultural workers from health hazards and provide a basis for risk assessment.

Original languageEnglish
Pages (from-to)55-59
Number of pages5
JournalEnvironmental and Molecular Mutagenesis
Volume31
Issue number1
DOIs
Publication statusPublished - 1998

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Keywords

  • P-postlabeling
  • DNA adducts
  • Herbicide
  • Roundup

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Genetics
  • Genetics(clinical)
  • Toxicology
  • Health, Toxicology and Mutagenesis

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