TY - JOUR
T1 - 3,4-Dichloroaniline-haemoglobin adducts in humans
T2 - Preliminary data on agricultural workers exposed to propanil
AU - Pastorelli, Roberta
AU - Catenacci, Giovanni
AU - Guanci, Marco
AU - Fanelli, Roberto
AU - Valoti, Elio
AU - Minoia, Claudio
AU - Airoldi, Luisa
PY - 1998/5
Y1 - 1998/5
N2 - Propanil is one of the major herbicides used on rice-paddies and is thought to produce adverse health effects through the action of its metabolite 3,4-dichloroaniline (3,4-DCA). The feasibility of monitoring human exposure to propanil on the basis of 3,4-DCA adducts to haemoglobin (Hb) was investigated. We developed a method based on gas chromatography negative ion chemical ionization-mass spectrometry (NICI-GC-MS) to quantify 3,4-DCA released from human Hb after alkaline hydrolysis of the protein. 3,4-DCA-Hb adducts were identified in agricultural workers exposed to propanil and were detectable even 4 months after the last herbicide application. Urine samples collected at the same time had no measurable level of 3,4-DCA. 3,4-DCA-Hb adducts might be useful for monitoring human exposure to 3,4-DCA from agricultural sources.
AB - Propanil is one of the major herbicides used on rice-paddies and is thought to produce adverse health effects through the action of its metabolite 3,4-dichloroaniline (3,4-DCA). The feasibility of monitoring human exposure to propanil on the basis of 3,4-DCA adducts to haemoglobin (Hb) was investigated. We developed a method based on gas chromatography negative ion chemical ionization-mass spectrometry (NICI-GC-MS) to quantify 3,4-DCA released from human Hb after alkaline hydrolysis of the protein. 3,4-DCA-Hb adducts were identified in agricultural workers exposed to propanil and were detectable even 4 months after the last herbicide application. Urine samples collected at the same time had no measurable level of 3,4-DCA. 3,4-DCA-Hb adducts might be useful for monitoring human exposure to 3,4-DCA from agricultural sources.
KW - Biomonitoring
KW - Haemoglobin adducts
KW - Propanil
UR - http://www.scopus.com/inward/record.url?scp=0031781572&partnerID=8YFLogxK
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U2 - 10.1080/135475098231246
DO - 10.1080/135475098231246
M3 - Article
C2 - 23899332
AN - SCOPUS:0031781572
VL - 3
SP - 227
EP - 233
JO - Biomarkers
JF - Biomarkers
SN - 1354-750X
IS - 3
ER -