-374T/A polymorphism of the RAGE gene promoter in relation to severity of coronary atherosclerosis

Colomba Falcone, Ilaria Campo, Enzo Emanuele, Maria P. Buzzi, Diego Geroldi, Chiara Belvito, Michele Zorzetto, Ilaria Sbarsi, Mariaclara Cuccia

Research output: Contribution to journalArticle

Abstract

Background: We have recently reported that homozygosity for the minor A-allele of RAGE (receptor for advanced glycation end products) -374T/A polymorphism may exert a protective effect toward the development of angiographic coronary artery disease (CAD). Here we focused on the putative involvement of this functional RAGE polymorphism on the severity of coronary atherosclerosis as assessed by angiography. Methods: In a total of 234 consecutive Caucasian patients with angiographically proven CAD, the severity of coronary atherosclerosis was assessed by the number of diseased vessels (greater than 50% stenosis). Genotyping for the -374T/A variant was performed by means of PCR-RFLPs. Results: The mean number of diseased vessels was significantly lower in patients with the AA genotype (1.47±0.68) than in those with the AT or TT genotype (1.88±0.82, p=0.029). After confounding variables were controlled for, the number of diseased vessels remained significantly different in the AA genotype carriers from that in the AT or TT carriers (p=0.041, ANCOVA). Conclusions: Our data suggest that the RAGE -374T/A polymorphism is one of the likely candidate determinants for the genetic variance of disease phenotype in coronary atherosclerosis.

Original languageEnglish
Pages (from-to)111-116
Number of pages6
JournalClinica Chimica Acta
Volume354
Issue number1-2
DOIs
Publication statusPublished - Apr 2005

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Keywords

  • Angiography
  • Coronary artery disease
  • Disease severity
  • Receptor for advanced glycation end products
  • Single nucleotide polymorphism

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

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