3D gelatin-chitosan hybrid hydrogels combined with human platelet lysate highly support human mesenchymal stem cell proliferation and osteogenic differentiation

Federica Re, Luciana Sartore, Vladimira Moulisova, Marco Cantini, Camillo Almici, Andrea Bianchetti, Clizia Chinello, Kamol Dey, Silvia Agnelli, Cristina Manferdini, Simona Bernardi, Nicola F Lopomo, Emilio Sardini, Elisa Borsani, Luigi F Rodella, Fabio Savoldi, Corrado Paganelli, Pierangelo Guizzi, Gina Lisignoli, Fulvio Magni & 2 others Manuel Salmeron-Sanchez, Domenico Russo

Research output: Contribution to journalArticle

Abstract

Bone marrow and adipose tissue human mesenchymal stem cells were seeded in highly performing 3D gelatin-chitosan hybrid hydrogels of varying chitosan content in the presence of human platelet lysate and evaluated for their proliferation and osteogenic differentiation. Both bone marrow and adipose tissue human mesenchymal stem cells in gelatin-chitosan hybrid hydrogel 1 (chitosan content 8.1%) or gelatin-chitosan hybrid hydrogel 2 (chitosan 14.9%) showed high levels of viability (80%-90%), and their proliferation and osteogenic differentiation was significantly higher with human platelet lysate compared to fetal bovine serum, particularly in gelatin-chitosan hybrid hydrogel 1. Mineralization was detected early, after 21 days of culture, when human platelet lysate was used in the presence of osteogenic stimuli. Proteomic characterization of human platelet lysate highlighted 59 proteins mainly involved in functions related to cell adhesion, cellular repairing mechanisms, and regulation of cell differentiation. In conclusion, the combination of our gelatin-chitosan hybrid hydrogels with hPL represents a promising strategy for bone regenerative medicine using human mesenchymal stem cells.

Original languageEnglish
Pages (from-to)1-16
Number of pages16
JournalJournal of Tissue Engineering
Volume10
DOIs
Publication statusPublished - May 21 2019

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Hydrogels
Cell proliferation
Chitosan
Gelatin
Platelets
Stem cells
Mesenchymal Stromal Cells
Blood Platelets
Cell Proliferation
Hydrogel
Bone
Bone and Bones
Adipose Tissue
Bone Marrow
Tissue
Regenerative Medicine
Cell adhesion
Cell Adhesion
Proteomics
Cell Differentiation

Keywords

  • Hybrid chitosan-gelatin hydrogel
  • bone regeneration
  • human mesenchymal stem cells
  • human platelet lysate
  • tissue engineering

Cite this

3D gelatin-chitosan hybrid hydrogels combined with human platelet lysate highly support human mesenchymal stem cell proliferation and osteogenic differentiation. / Re, Federica; Sartore, Luciana; Moulisova, Vladimira; Cantini, Marco; Almici, Camillo; Bianchetti, Andrea; Chinello, Clizia; Dey, Kamol; Agnelli, Silvia; Manferdini, Cristina; Bernardi, Simona; Lopomo, Nicola F; Sardini, Emilio; Borsani, Elisa; Rodella, Luigi F; Savoldi, Fabio; Paganelli, Corrado; Guizzi, Pierangelo; Lisignoli, Gina; Magni, Fulvio; Salmeron-Sanchez, Manuel; Russo, Domenico.

In: Journal of Tissue Engineering, Vol. 10, 21.05.2019, p. 1-16.

Research output: Contribution to journalArticle

Re, F, Sartore, L, Moulisova, V, Cantini, M, Almici, C, Bianchetti, A, Chinello, C, Dey, K, Agnelli, S, Manferdini, C, Bernardi, S, Lopomo, NF, Sardini, E, Borsani, E, Rodella, LF, Savoldi, F, Paganelli, C, Guizzi, P, Lisignoli, G, Magni, F, Salmeron-Sanchez, M & Russo, D 2019, '3D gelatin-chitosan hybrid hydrogels combined with human platelet lysate highly support human mesenchymal stem cell proliferation and osteogenic differentiation', Journal of Tissue Engineering, vol. 10, pp. 1-16. https://doi.org/10.1177/2041731419845852
Re, Federica ; Sartore, Luciana ; Moulisova, Vladimira ; Cantini, Marco ; Almici, Camillo ; Bianchetti, Andrea ; Chinello, Clizia ; Dey, Kamol ; Agnelli, Silvia ; Manferdini, Cristina ; Bernardi, Simona ; Lopomo, Nicola F ; Sardini, Emilio ; Borsani, Elisa ; Rodella, Luigi F ; Savoldi, Fabio ; Paganelli, Corrado ; Guizzi, Pierangelo ; Lisignoli, Gina ; Magni, Fulvio ; Salmeron-Sanchez, Manuel ; Russo, Domenico. / 3D gelatin-chitosan hybrid hydrogels combined with human platelet lysate highly support human mesenchymal stem cell proliferation and osteogenic differentiation. In: Journal of Tissue Engineering. 2019 ; Vol. 10. pp. 1-16.
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AU - Sartore, Luciana

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AU - Cantini, Marco

AU - Almici, Camillo

AU - Bianchetti, Andrea

AU - Chinello, Clizia

AU - Dey, Kamol

AU - Agnelli, Silvia

AU - Manferdini, Cristina

AU - Bernardi, Simona

AU - Lopomo, Nicola F

AU - Sardini, Emilio

AU - Borsani, Elisa

AU - Rodella, Luigi F

AU - Savoldi, Fabio

AU - Paganelli, Corrado

AU - Guizzi, Pierangelo

AU - Lisignoli, Gina

AU - Magni, Fulvio

AU - Salmeron-Sanchez, Manuel

AU - Russo, Domenico

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N2 - Bone marrow and adipose tissue human mesenchymal stem cells were seeded in highly performing 3D gelatin-chitosan hybrid hydrogels of varying chitosan content in the presence of human platelet lysate and evaluated for their proliferation and osteogenic differentiation. Both bone marrow and adipose tissue human mesenchymal stem cells in gelatin-chitosan hybrid hydrogel 1 (chitosan content 8.1%) or gelatin-chitosan hybrid hydrogel 2 (chitosan 14.9%) showed high levels of viability (80%-90%), and their proliferation and osteogenic differentiation was significantly higher with human platelet lysate compared to fetal bovine serum, particularly in gelatin-chitosan hybrid hydrogel 1. Mineralization was detected early, after 21 days of culture, when human platelet lysate was used in the presence of osteogenic stimuli. Proteomic characterization of human platelet lysate highlighted 59 proteins mainly involved in functions related to cell adhesion, cellular repairing mechanisms, and regulation of cell differentiation. In conclusion, the combination of our gelatin-chitosan hybrid hydrogels with hPL represents a promising strategy for bone regenerative medicine using human mesenchymal stem cells.

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