[3H]Norepinephrine release from hippocampal slices is an in vitro biochemical tool for investigating the pharmacological properties of excitatory amino acid receptors

A. Vezzani, H. Q. Wu, R. Samanin

Research output: Contribution to journalArticle

Abstract

[3H]Norepinephrine ([3H]NE) efflux from preloaded rat hippocampal slices was increased in a dose-dependent manner by excitatory amino acids, with the following order of potencies: N-methyl-D-aspartate (NMDA) > kainic acid (KA) > L-glutamate ≥ D,L-homocysteate > L-aspartate > quinolinic acid > quisqualic acid. The effect of the excitatory amino acids was blocked by physiological concentrations of Mg2+, with the exception of KA. D,L-2-Amino-7-phosphonoheptanoic acid dose-dependently inhibited the NMDA effect (ID50 = 69 μM), whereas at 1 mM it was ineffective versus KA. The release of [3H]NE induced by quinolinic acid was blocked by 0.1 mM D,L-2-amino-7-phosphonoheptanoic acid. γ-D-Glutamylglycine dose-dependently inhibited the KA effect with an ID50 of 1.15 mM. Tetrodotoxin (2 μM) reduced by 40 and 20% the NMDA and KA effects, respectively. The data indicate that [3H]NE release from hippocampal slices can be used as a biochemical marker for pharmacological investigations of excitatory amino acid receptors and their putative agonists and antagonists.

Original languageEnglish
Pages (from-to)1438-1442
Number of pages5
JournalJournal of Neurochemistry
Volume49
Issue number5
Publication statusPublished - 1987

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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